Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
FAM148C CRISPR/Cas9 KO Plasmid (h): sc-414279
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- FAM148C CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the FAM148C genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: FAM148C Antibody (F-10): sc-515089
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FAM148C CRISPR/Cas9 KO Plasmid (h) | sc-414279 | 20 µg | $397.00 |
Overview
C2CD4C (FAM148C) encodes a C2 domain–containing protein implicated in calcium-dependent membrane-associated signaling and regulation of protein–protein interactions at intracellular membranes. Emerging functional annotations link FAM148C to pathways coordinating vesicular trafficking, cytoskeletal dynamics, and stimulus-responsive transcriptional programs that influence cell growth and metabolic homeostasis. Genetic studies have associated variation in the C2CD4C locus with glycemic traits and type 2 diabetes risk, supporting investigation of its roles in pancreatic islet function and broader endocrine signaling networks. These features make FAM148C a useful target for mechanistic studies of signaling integration and genotype-to-phenotype relationships in human cell models.
FAM148C CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the C2CD4C gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the C2CD4C together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the C2CD4C open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish FAM148C protein expression.
This CRISPR knockout system enables efficient generation of C2CD4C-deficient cell models for investigation of FAM148C signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting C2CD4C exon(s) critical for FAM148C function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple C2CD4C genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by FAM148C CRISPR/Cas9 KO Plasmid (h) and FAM148C CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the C2CD4C locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by FAM148C HDR Plasmid (h) and FAM148C HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by C2CD4C homology arms to support homology-directed repair at defined C2CD4C target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.