EphB3 항체 (7E5) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 EphB3을 검출할것을 권장합니다.

EphB3 항체 (7E5): sc-100299

EphB3 항체 (7E5) is rated 4.6 out of 5 by 5.
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Datasheets

    • EphB3 항체(7E5) 는 마우스 monoclonal IgG1(kappa light chain) 이며 100 µg/ml으로 제공합니다.
    • human origin의 EphB3의 전체인 899-998 아미노산을 항원으로 사용하였습니다.
    • WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 EphB3을 검출할것을 권장합니다.
    • 강력추천하는 conventional secondary anti-mouse IgG reagents의 대체제품 보기m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP).

Can sc-100299: EphB3 (7E5) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: cjMara
Thank you for your question. Yes, sc-100299: EphB3 (7E5) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Produced good immunoperoxidase membrane staining Produced good immunoperoxidase membrane staining in formalin-fixed, paraffin-embedded human tonsil tissue. -SCBT QC
Date published: 2015-04-16
Rated 4 out of 5 by from Positive detection of endogenous EphB3 protein Positive detection of endogenous EphB3 protein by WB using HeLa whole cell lysate. -SCBT QC
Date published: 2015-03-04
Rated 5 out of 5 by from Publishable data obtained by WB and IHC using Publishable data obtained by WB and IHC using mouse bone marrow. -SCBT Publication Review
Date published: 2015-02-16
Rated 5 out of 5 by from Good specificity shown by using transfected Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the human EphB3 protein via Western Blot analysis. -SCBT QC
Date published: 2015-02-03
Rated 5 out of 5 by from An increase in signal was observed via Western An increase in signal was observed via Western Blot when comparing mouse EphB3 transfected 293T cells with those that have not been transfected to over express the protein of interest. -SCBT QC
Date published: 2015-01-19
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