EPAS-1 항체 (190b) WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 EPAS-1을 검출할것을 권장합니다.

EPAS-1 항체 (190b): sc-13596

EPAS-1 항체 (190b) is rated 4.0 out of 5 by 9.
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데이터 시트

    • 안티-EPAS-1 항체 190b 는 마우스 monoclonal IgG1 (kappa light chain) EPAS-1 항체 이며 200 µg/ml으로 제공합니다.
    • human origin의 EPAS-1의 전체인 아미노산을 항원으로 사용하였습니다.
    • 안티-EPAS-1 항체 (190b)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 EPAS-1 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-EPAS-1 항체 (190b)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-13596 X, 200 µg/0.1 ml)이 있습니다.
    • 강력추천하는 conventional secondary anti-mouse IgG reagents의 대체제품 보기m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP).
    • EPAS-1 (190b): sc-13596무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.

What is recommended incubation time for western blot of cell extracts with sc-13596? Thank You.

Asked by: KateDeck
Thank you for your question. We recommend incubating the primary antibody for 1 hour at room temperature (or overnight at 4C) in 5% nonfat milk in TBST.
Answered by: Technical Support
Date published: 2019-08-17

When should the TransCruz reagent sc-13596 X be used instead of the standard form sc-13596? What is the difference between the two forms?

Asked by: TinTin
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration. The TransCruz reagent is provided as 10x more concentrated than the regular form: sc-13596 X, 200 µg/0.1 ml.
Answered by: Technical Support 9
Date published: 2017-01-12

Can this antibody be used for ChIP?

Asked by: TinTin
Thank you for your question! Yes, this antibody is recommeded for IP, which includes ChIP and CoIP. For more information, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Technical Support 9
Date published: 2017-01-12
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Rated 4 out of 5 by from 良いです。 内在性タンパク質を検出できますが、そのためには1次抗体をCanget signalで希釈する必要があります。
Date published: 2020-10-20
Rated 1 out of 5 by from 在小鼠肾脏组织中没有杂到目标条带。 这抗体在肾脏组织中没有杂到目标条带,在70KD以上都没有任何条带,但在50~30KD之间有许多杂带,。
Date published: 2017-11-27
Rated 5 out of 5 by from Great I am happy to get chance to try it. Will let you know how it is in rat sample.
Date published: 2017-07-05
Rated 4 out of 5 by from good band. Band had seen in hypoxic condition at 1:200 dilution in western blot.
Date published: 2017-06-16
Rated 4 out of 5 by from Buon funzionamento Sebbene scettica ho provato che questo anticorpo funziona
Date published: 2017-03-22
Rated 5 out of 5 by from Strong western data Very clear western blot data; strong signal with low background
Date published: 2017-01-31
Rated 5 out of 5 by from Good choice for WB Whether your samples are mouse, rat or human, sc-13596 will provide you with strong and clear bands. This antibody has been published nearly 20 times.
Date published: 2017-01-30
Rated 4 out of 5 by from Strong band seen in WB using CoCl2 Strong band seen in WB using CoCl2-induced HeLa whole cell lysate. -SCBT QC
Date published: 2015-03-10
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