Electrophoresis Sample Buffer, 2X A ready-to-use reducing sample buffer for SDS-PAGE.

Electrophoresis Sample Buffer, 2X

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Electrophoresis Sample Buffer, 2X is rated 4.22 out of 5 by 9.
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Alternate Names: Electrophoresis Sample Buffer, 2X is known as the most common SDS-PAGE sample buffer.
Application Electrophoresis Sample Buffer, 2X is a ready-to-use reducing sample buffer for SDS-PAGE, acting on denatured proteins in the system.
연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.
* 참조분석증명서대량의 측정 데이터(함수량포함).
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Electrophoresis Sample Buffer, 2X is a ready-to-use reducing electrophoresis sample buffer solution with bromophenol blue for the preparation of protein samples to be separated in SDS-PAGE. Bromophenol Blue is included with Electrophoresis Sample Buffer, 2X as a tracking dye to monitor the sample during the electrophoretic run. Electrophoresis Sample Buffer, 2X contains DTT (sc-29089), which disrupts disulfide bonds to ensure proteins are fully denatured before loading on the gel. Electrophoresis Sample Buffer, 2X also contains glycerol, TCEP, DTT, SDS, 1.0M Tris-HCl pH 6.7 and bromophenol blue. For non-reducing SDS-PAGE, Electrophoresis Sample Buffer 2X (non-reducing) (sc-45085) is recommended.


References

1. Toyoda, E. et al. 2005. Pancreas. 30(2): 168-173. PMID: 15714139
2. Heinz, L.X. et al. 2006. Blood. 107(4): 1445-1453. PMID: 16223775
3. Gohil, K. et al. 2008. Brain Res. 1201 : 167-176. PMID: 18299118
4. Popovici, R.M. et al. 2008. Endocrinology. 149(3): 1136-1143. PMID: 18048494
5. Tang, Y. et al. 2010. J. Nutr. Biochem. 21(3): 237-246. PMID: 19369054

사용법 :
Mix sample with an equal volume of Electrophoresis Sample Buffer, 2X. Bring to 95° C for 10 minutes, cool to room temperature before loading. If particulate is present, centrifuge samples 5 minutes at 14k RPM in microcentrifuge, and load the gel.
Appearance :
Aqueous solution
Physical State :
Liquid
저장 :
Store at 4° C
연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

SDS(MSDS)다운로드

분석증명서

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Electrophoresis Sample Buffer, 2X  논문정보

다른 사용자의 Electrophoresis Sample Buffer, 2X사용법보기. 클릭하여 PubMed entry보기 .

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PMID: # 34142127  Rios, JJ.|Denton, K.|Yu, H.|Manickam, K.|Garner, S.|Russell, J.|Ludwig, S.|Rosenfeld, JA.|Liu, P.|Munch, J.|Sucato, DJ.|Beutler, B.|Wise, CA.| et al. 2021. Dis Model Mech. 14:

PMID: # 34079997  Rios, JJ.|Denton, K.|Yu, H.|Manickam, K.|Garner, S.|Russell, J.|Ludwig, S.|Rosenfeld, JA.|Liu, P.|Munch, J.|Sucato, DJ.|Beutler, B.|Wise, CA.| et al. 2021. Dis Model Mech.

PMID: # 29326957  Garrido, D. et al. 2017. Front Vet Sci. 4: 226.

PMID: # 26514550  Winiarska, K. et al. 2016. Journal of pineal research. 60: 109-17.

PMID: # 26432891  Talaei, N. et al. 2015. J. Immunol. 195: 4623-31.

PMID: # 26734660  Peng, S. et al. 2015. Annals of clinical and translational neurology. 2: 1085-104.

PMID: # 24370615  Joiner, DM. et al. 2014. Calcified tissue international. 94: 484-94.

PMID: # 23686888  Jia, Y. et al. 2013. Andrology. 1: 651-9.

PMID: # 23787122  Kaufman, JL. et al. 2013. Clinical lymphoma, myeloma & leukemia. 13: 370-6.

PMID: # 23291594  Wang, R. et al. 2013. Free radical biology & medicine. 57: 141-53.

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