Date published: 2026-5-24

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eIF5 Antibody (20): sc-135894

5.0(2)
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  • eIF5 Antibody (20) is a mouse monoclonal IgG1, cited in 2 publications, provided at 50 µg/500 µl
  • raised against amino acids 100-215 of eIF5 of human origin
  • recommended for detection of eIF5 of mouse, rat and human origin by WB, IP and IF
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for eIF5 Antibody (20). This work is in progress.
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eIF5 Antibody (20) is a mouse monoclonal IgG1 antibody that detects eIF5 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). eIF5 plays a crucial role in the regulation of translation initiation, specifically by catalyzing the hydrolysis of GTP bound to the 40S ribosomal subunit, which is essential for the assembly of the 80S initiation complex. This process is vital for the accurate and efficient translation of mRNA into proteins, impacting cellular function and growth. Dysregulation of eIF5 and other initiation factors can lead to aberrant protein synthesis, contributing to various diseases, including cancer. Anti-eIF5 antibody (20) provides reliable detection of eIF5, enabling researchers to explore its role in translation initiation and its implications in disease mechanisms. eIF5 (20) monoclonal antibody serves as an invaluable tool for studies focused on understanding the complexities of protein synthesis and the potential therapeutic targets within this pathway.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

eIF5 Antibody (20)

sc-135894
50 µg/500 µl
$322.00

I am using eIF5 (20): sc-135894 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Germaine
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
  • y_2026, m_5, d_24, h_14CST
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Rated 5 out of 5 by from Worked well in IF in WIWorked well in IF in WI-38 cells and showed strong nuclear localization. -SCBT QC
Date published: 2015-05-11
Rated 5 out of 5 by from Strong band in WB in Hep G2 whole cell lysateStrong band in WB in Hep G2 whole cell lysate. -SCBT QC
Date published: 2013-10-28
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eIF5 Antibody (20) is rated 5.0 out of 5 by 2.
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