Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
EF-2 CRISPR/Cas9 KO Plasmid (m): sc-420117
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EF-2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the EF-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EF-2 Antibody (C-9): sc-166415
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EF-2 CRISPR/Cas9 KO Plasmid (m) | sc-420117 | 20 µg | $397.00 |
Overview
Eef2 encodes eukaryotic elongation factor 2 (EF-2), a conserved GTPase that drives ribosomal translocation during mRNA translation elongation and thereby controls global protein synthesis rates. EF-2 activity is tightly regulated by phosphorylation via eEF2 kinase downstream of nutrient- and stress-sensing pathways, including mTOR signaling, linking translational output to energy status, hypoxia, and synaptic activity. Through its central role in proteostasis, EF-2 influences cell growth, stress responses, and differentiation programs that depend on rapid remodeling of the proteome. Dysregulated translational control involving EF-2 and eEF2K has been implicated in neurological dysfunction and tumor-associated metabolic adaptation, making Eef2 a useful node for mechanistic studies of translational regulation in mouse systems.
EF-2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Eef2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Eef2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Eef2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish EF-2 protein expression.
This CRISPR knockout system enables efficient generation of Eef2-deficient cell models for investigation of EF-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Eef2 exon(s) critical for EF-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Eef2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by EF-2 CRISPR/Cas9 KO Plasmid (m) and EF-2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Eef2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by EF-2 HDR Plasmid (m) and EF-2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Eef2 homology arms to support homology-directed repair at defined Eef2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.