ECM29 Double Nickase Plasmid (h): sc-409848-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ECM29 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ECM29 Double Nickase Plasmid (h) and ECM29 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KIAA0368. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ECM29 Double Nickase Plasmid (h) | sc-409848-NIC | 20 µg | $410.00 |
KIAA0368 encodes ECM29, a large scaffold protein that associates with the 26S proteasome and helps coordinate proteasome assembly, localization, and quality control. By modulating proteasome dynamics, ECM29 influences ubiquitin-dependent protein turnover and intersects with proteostasis pathways that govern cell-cycle progression, DNA damage responses, and stress signaling. Altered proteasome regulation has been linked to dysregulated degradation of key signaling proteins and broader disturbances in cellular homeostasis relevant to cancer biology and neurodegeneration research. As a result, ECM29 is commonly studied in contexts where proteasome function, ubiquitination, and cellular stress adaptation are central experimental variables.
ECM29 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KIAA0368 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KIAA0368. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KIAA0368 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KIAA0368-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.