E2A Antibody (G-9): sc-365261
- E2A Antibody (G-9) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 208-649 mapping within a basic helix-loop-helix domain of E12 (corresponding to amino acids 208-649 of E47) of human origin
- recommended for detection of E2A isoforms E12 and E47 of mouse, rat and human origin by WB, IP, IF and ELISA
- TransCruz reagent for ChIP application (sc-365261 X, 200 µg/0.1 ml)
- See E2A (G-2): sc-133075 for E2A antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for E2A Antibody (G-9) for WB applications. These reagents are now offered in bundles with E2A Antibody (G-9) (see ordering information below).
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E2A Antibody (G-9) is a mouse monoclonal IgG1 antibody that detects E2A in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). E2A, a member of the basic helix-loop-helix (bHLH) transcription factor family, plays a crucial role in regulating gene expression during B cell maturation and myogenic differentiation. E2A anti-E2A antibody (G-9) targets proteins primarily located in the nucleus, where E2A binds to specific DNA sequences to regulate transcription of target genes essential for cell differentiation and development. Nuclear localization of E2A enables direct interaction with DNA and other transcriptional regulators, thereby influencing expression of genes involved in various cellular processes, including development of muscle cells and B lymphocytes. E2A forms heterodimers with other bHLH proteins, such as Myo D, which enhances binding to E box motifs in promoter regions of muscle-specific genes, underscoring E2A′s importance in lineage-specific gene expression. Anti-E2A antibody (G-9) serves as an invaluable tool for researchers studying intricate mechanisms of gene regulation and cellular differentiation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
E2A Antibody (G-9) References:
- E2A proteins: essential regulators at multiple stages of B-cell development. | Kee, BL., et al. 2000. Immunol Rev. 175: 138-49. PMID: 10933599
- E2A-HLF usurps control of evolutionarily conserved survival pathways. | Seidel, MG. and Look, AT. 2001. Oncogene. 20: 5718-25. PMID: 11607821
- Regulation and function of the E2A proteins in B cell development. | Murre, C. 2007. Adv Exp Med Biol. 596: 1-7. PMID: 17338171
- E2A predicts prognosis of colorectal cancer patients and regulates cancer cell growth by targeting miR-320a. | Huang, A., et al. 2014. PLoS One. 9: e85201. PMID: 24454819
- Direct and indirect targets of the E2A-PBX1 leukemia-specific fusion protein. | Diakos, C., et al. 2014. PLoS One. 9: e87602. PMID: 24503810
- E2a is necessary for Smad2/3-dependent transcription and the direct repression of lefty during gastrulation. | Wills, AE. and Baker, JC. 2015. Dev Cell. 32: 345-57. PMID: 25669884
- E2A-PBX1 Remodels Oncogenic Signaling Networks in B-cell Precursor Acute Lymphoid Leukemia. | Duque-Afonso, J., et al. 2016. Cancer Res. 76: 6937-6949. PMID: 27758892
- Expression of E2A in mid-secretory endometrium of women suffering from recurrent miscarriage. | Yin, ZN., et al. 2017. J Huazhong Univ Sci Technolog Med Sci. 37: 910-914. PMID: 29270752
- E2A ablation enhances proportion of nodal-like cardiomyocytes in cardiac-specific differentiation of human embryonic stem cells. | Li, X., et al. 2021. EBioMedicine. 71: 103575. PMID: 34488017
- E2A-HLF chimeric transcription factors in pro-B cell acute lymphoblastic leukemia. | Look, AT. 1997. Curr Top Microbiol Immunol. 220: 45-53. PMID: 9103674
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
E2A Antibody (G-9) | sc-365261 | 200 µg/ml | $322.00 | |||
E2A Antibody (G-9): m-IgG Fc BP-HRP Bundle | sc-540323 | 200 µg Ab; 10 µg BP | $361.00 | |||
E2A Antibody (G-9): m-IgG1 BP-HRP Bundle | sc-542041 | 200 µg Ab; 20 µg BP | $361.00 | |||
E2A Antibody (G-9) X | sc-365261 X | 200 µg/0.1 ml | $322.00 |