Dynactin p62 Antibody (H-4): sc-55603

Dynactin p62 Antibody (H-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects Dynactin p62 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Dynactin p62 antibody (H-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dynactin p62 plays a crucial role in cellular processes powered by the microtubule-based motor cytoplasmic dynein, serving as a vital cofactor in these mechanisms. Dynactin p62 functions as a key component of the dynactin complex, which is essential for proper dynein function, facilitating intracellular transport and organelle movement. Dynactin p62 contains a highly conserved cysteine-rich motif that directly interacts with actin-related protein 1 (Arp1), contributing to the structural integrity and functionality of the dynactin complex. Dynactin p62 exhibits a punctate cytoplasmic distribution and localizes to the centrosome, with significant expression levels in the nucleus. This distribution pattern suggests a multifaceted role in cellular dynamics, linking dynein and dynactin to the cortical cytoskeleton and influencing various cellular processes, including mitosis and organelle positioning.

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Dynactin p62 Antibody (H-4) References:

1. Self-regulated polymerization of the actin-related protein Arp1.  |  Bingham, JB. and Schroer, TA. 1999. Curr Biol. 9: 223-6. PMID: 10074429
2. Analysis of dynactin subcomplexes reveals a novel actin-related protein associated with the arp1 minifilament pointed end.  |  Eckley, DM., et al. 1999. J Cell Biol. 147: 307-20. PMID: 10525537
3. Interaction of the p62 subunit of dynactin with Arp1 and the cortical actin cytoskeleton.  |  Garces, JA., et al. Curr Biol. 9: 1497-500. PMID: 10607597
4. A dynactin subunit with a highly conserved cysteine-rich motif interacts directly with Arp1.  |  Karki, S., et al. 2000. J Biol Chem. 275: 4834-9. PMID: 10671518
5. Immunolocalization of cytoplasmic dynein and dynactin subunits in cultured macrophages: enrichment on early endocytic organelles.  |  Habermann, A., et al. 2001. J Cell Sci. 114: 229-240. PMID: 11112706
6. Copper-dependent interaction of dynactin subunit p62 with the N terminus of ATP7B but not ATP7A.  |  Lim, CM., et al. 2006. J Biol Chem. 281: 14006-14. PMID: 16554302
7. The p25 subunit of the dynactin complex is required for dynein-early endosome interaction.  |  Zhang, J., et al. 2011. J Cell Biol. 193: 1245-55. PMID: 21708978
8. Dynactin helps target Polo-like kinase 1 to kinetochores via its left-handed beta-helical p27 subunit.  |  Yeh, TY., et al. 2013. EMBO J. 32: 1023-35. PMID: 23455152
9. Cryo-EM reveals the complex architecture of dynactin's shoulder region and pointed end.  |  Lau, CK., et al. 2021. EMBO J. 40: e106164. PMID: 33734450
10. Mystery of methamphetamine-induced autophagosome accumulation in hippocampal neurons: loss of syntaxin 17 in defects of dynein-dynactin driving and autophagosome-late endosome/lysosome fusion.  |  Xu, H., et al. 2021. Arch Toxicol. 95: 3263-3284. PMID: 34374793
11. Ultrastructural analysis of the dynactin complex: an actin-related protein is a component of a filament that resembles F-actin.  |  Schafer, DA., et al. 1994. J Cell Biol. 126: 403-12. PMID: 7518465

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Dynactin p62 Antibody (H-4) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-Dynactin p62 Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Dynactin p62 Antibody (H-4) Specifications

Alternate Product Name: Dynactin p62

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 161-460

Epitope Target: Dynactin p62

Epitope Species: human

Product Reactivity/Detection: Dynactin p62

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for Dynactin p62 Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin