Date published: 2025-9-6

001 800-1338-3838

SCBT Portrait Logo
Seach Input

Doublecortin/DCX 항체 (E-6): sc-271390

4.3(39)
리뷰 쓰기질문하기

데이터 시트
  • Doublecortin/DCX 항체 E-6 는 마우스 monoclonal IgG1 κ Doublecortin/DCX 항체, 141간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • human origin의 Doublecortin을 항원으로 사용하였습니다.
  • Doublecortin/DCX 항체 (E-6)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 Doublecortin 를 감지하는 데에 추천한다.
  • 항-Doublecortin/DCX 항체(E-6)는 IP용 agarose와 결합되어 이용 가능하며, WB, IHC(P), ELISA용 HRP와 결합되어 이용 가능하며, IF, IHC(P), FCM용 phycoerythrin 또는 FITC와 결합되어 이용 가능합니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • Doublecortin/DCX (E-6): sc-271390무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 는 Doublecortin/DCX 항체 (E-6) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은Doublecortin/DCX 항체(E-6) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다)

빠른 링크

더보기

더블코르틴 항체(E-6)는 마우스, 쥐 및 인간 유래의 더블코르틴 단백질을 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgG1 κ 마우스 단일 클론 더블코르틴 항체(DCX 항체로도 지정됨)입니다. 더블코틴 항체(E-6)는 비접합 항-더블코틴 항체 형태와 아가로스, HRP, PE, FITC 및 다중 Alexa Fluor® 접합체를 포함한 여러 접합 형태의 항-더블코틴 항체 형태로 제공됩니다. 리센슈탈리(매끄러운 뇌)는 불완전한 신경세포 이동과 매끄러운 대뇌 표면을 특징으로 하는 뇌 발달 이상으로, 심각한 정신 지체를 초래합니다. 유전자 분석 결과 일부 리센팔리아에서 돌연변이가 발생하는 두 가지 단백질, 즉 리센팔리아-1 단백질(LIS1)과 더블코틴이 확인되었습니다. LIS1은 이종 삼중체 G 단백질의 β- 서브유닛과 서열 상동성을 보입니다. 더블코틴은 일치하는 Abl 인산화 부위를 포함하고 있으며, 예측된 키나아제 단백질과 일부 서열 상동성을 가지고 있습니다. 두 단백질 모두 발달 중인 뇌에서 높게 발현되며, 이는 두 단백질이 뇌 발달에 중요한 신호 전달 경로에 관여할 수 있음을 시사합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

Doublecortin/DCX 항체 (E-6) 참고문헌:

  1. 뉴로파신의 세포질 영역에서 포스포-피그큐이 티로신을 인식하는 더블코틴의 새로운 활성.  |  Kizhatil, K., et al. 2002. J Neurosci. 22: 7948-58. PMID: 12223548
  2. 발달 중인 쥐 망막에서 더블코틴의 차별적 발현 및 세포 내 국소화.  |  Lee, EJ., et al. 2003. Eur J Neurosci. 17: 1542-8. PMID: 12752371
  3. 중추 및 말초 신경계 종양과 인간 비신경 조직에서 더블코틴의 발현.  |  Bernreuther, C., et al. 2006. Acta Neuropathol. 111: 247-54. PMID: 16520969
  4. 미세소관 세포 골격을 조절하는 더블코틴의 뚜렷한 역할.  |  Moores, CA., et al. 2006. EMBO J. 25: 4448-57. PMID: 16957770
  5. 쥐 망막의 수평 세포에서 더블코틴 발현은 성체기까지 지속됩니다.  |  Wakabayashi, T., et al. 2008. Neurosci Lett. 442: 249-52. PMID: 18647639
  6. 특정 항체와 복합된 인간 더블코틴 C- 및 N-말단 도메인의 결정 구조.  |  Burger, D., et al. 2016. J Biol Chem. 291: 16292-306. PMID: 27226599
  7. G 단백질 베타 서브유닛 유사 반복을 포함하는 밀러-디커 루게릭병 유전자의 분리.  |  Reiner, O., et al. 1993. Nature. 364: 717-21. PMID: 8355785
  8. WD-반복이 있는 단백질의 접기: WD-반복 슈퍼패밀리의 6가지 구성원과 G 단백질 베타 서브유닛의 비교.  |  Garcia-Higuera, I., et al. 1996. Biochemistry. 35: 13985-94. PMID: 8909296
  9. 혈소판 활성화 인자 아세틸하이드롤라제 발현과 활성은 신경세포 이동과 혈소판 활성화 인자 사이의 연관성을 시사합니다.  |  Albrecht, U., et al. 1996. Dev Biol. 180: 579-93. PMID: 8954729
  10. 신경세포 이동에 필요하고 X-연결 피질하 층상 이소성 및 리센슈탈 증후군에 관여하는 새로운 CNS 유전자입니다.  |  des Portes, V., et al. 1998. Cell. 92: 51-61. PMID: 9489699
  11. 더블코틴은 인간 X-연결성 리센셥증과 이중 피질 증후군에서 변이된 뇌 특이 유전자로, 추정 신호 단백질을 코딩합니다.  |  Gleeson, JG., et al. 1998. Cell. 92: 63-72. PMID: 9489700
  12. 리센 업!  |  Walsh, CA. 1998. Nat Genet. 19: 307-8. PMID: 9697681

주문정보

제품명카탈로그 번호 단위가격수량관심품목

Doublecortin/DCX 항체 (E-6)

sc-271390
200 µg/ml
$316.00

Doublecortin/DCX (E-6): m-IgG Fc BP-HRP 번들

sc-527240
200 µg Ab; 10 µg BP
$354.00

Doublecortin/DCX (E-6): m-IgG1 BP-HRP 번들

sc-532613
200 µg Ab; 20 µg BP
$354.00

Doublecortin/DCX 항체 (E-6) AC

sc-271390 AC
500 µg/ml, 25% agarose
$416.00

Doublecortin/DCX 항체 (E-6) HRP

sc-271390 HRP
200 µg/ml
$316.00

Doublecortin/DCX 항체 (E-6) FITC

sc-271390 FITC
200 µg/ml
$330.00

Doublecortin/DCX 항체 (E-6) PE

sc-271390 PE
200 µg/ml
$343.00

Doublecortin/DCX 항체 (E-6) Alexa Fluor® 488

sc-271390 AF488
200 µg/ml
$357.00

Doublecortin/DCX 항체 (E-6) Alexa Fluor® 546

sc-271390 AF546
200 µg/ml
$357.00

Doublecortin/DCX 항체 (E-6) Alexa Fluor® 594

sc-271390 AF594
200 µg/ml
$357.00

Doublecortin/DCX 항체 (E-6) Alexa Fluor® 647

sc-271390 AF647
200 µg/ml
$357.00

Doublecortin/DCX 항체 (E-6) Alexa Fluor® 680

sc-271390 AF680
200 µg/ml
$357.00

Doublecortin/DCX 항체 (E-6) Alexa Fluor® 790

sc-271390 AF790
200 µg/ml
$357.00

I've used DCX antibody E-6 and C-18 both. I've checked the DCX signal like branch shape in C-18 antibody, but I couldn't on E-6. so, i want to see some more pictures of DCX signal in brain. may I ?

Asked by: YAMKOOKIE
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-11-01

Do I need to use a "MOM" kit if using this product on mouse brain tissue?

Asked by: lee lee
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext. 1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-09-13

Is this antobody suitable for vibratome sections (not frozen)? Thank you !

Asked by: mmidroit
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

There was a mistake on the recipient and they put the Doublecortin Antibody (E-6) sc-271390 at -20 degrees for 3 hours. Does this affect the quality of the antibody or should it be fine?

Asked by: Anonymous
Thank you for your question. We do not recommend freezing our antibodies because they contain a small percentage of gelatin in the buffer as a stabilizing protein. Upon freezing, this can compromise the quality of the antibodies. However, if it was only frozen for 3 hours then we would still recommend testing the antibody because it may not have been compromised in such a short time.
Answered by: Technical Support
Date published: 2020-07-16

Why in the western blot image for mouse postnatal brain sample we see so many bands for doublecortin?

Asked by: Karishma
Thank you for your question, and for pointing attention to this image. Doublecortin is a hightly phosphorylated protein and in some samples it will detect post-translational modifications if they are present in the sample. We will take a look at this, it's a really strong and specific antibody for this target.
Answered by: Tech Service
Date published: 2020-02-17

Hello, please does this antibody react with avian species? Am working on Neurogenesis on the QUAIL brain. I saw that the older antibody goat anti-DCX C-18: SC-8066 react with avain species. DOES the goat anti-DCX (E-6): sc-271390 reacts with avians

Asked by: asamcy
Thank you for your question. Unfortunately we have not tested on bird samples, so it is not recommended or warrantied for that use. That said, we do have a publication in avian samples (chick, PMID: 25086319), so it very well may work fine for Quail samples.
Answered by: Tech Service
Date published: 2019-08-12

I wish to use this antibody (Doublecortin Antibody (E-6): sc-271390) against avian species. Can I get the information regarding the peptide sequence against which this antibody is designed?

Asked by: Pooja1
Thank you for your question. Unfortunately the exact epitope of this antibody has not been mapped, so I do not have that information. We do have a previous publication in avian samples (PMID: 25086319), maybe that is helpful for you.
Answered by: Tech Service
Date published: 2019-04-18

I am going to use Doublecortin (sc-271390 ) for IHC. my queries are 1. Will this be suitable for both IHC (paraformaldehyde fixed frozen sections) and Western blotting? 2. Could you suggest me a compatible secondary for this primary?

Asked by: Senthil
Thank you for your question. Yes, this antibody is recommended for both IHC on frozen sections and Western blot, so it is a great choice. I would recommend our new Mouse IgG binding protein for both WB and IHC, you can find them here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-130b1ry These are optimzed for these applications and we have had excellent results with them. We would be happy to send you samples of both the primary and the secondary reagents if you would like to try them! Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com for any sample requests or questions.
Answered by: Tech Service
Date published: 2017-05-29
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_10
  • loc_ko_KR, sid_271390, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 110ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from きれいに染色できましたラットの脳を用い、免疫組織化学染色 (IHC(P)) を行いました。 きれいに染色できたため今後も使用を検討したいと思います。
Date published: 2024-07-06
Rated 4 out of 5 by from Some lotes doesn't work!!!I bought a vial and it did work (lot C0118). A sample of the same cat. number given by a colleague worked fantastic. Just a comment on the picture you show, the red signal is mainly autofluorescence of blood. Attached is an image we obtained with the sample our colleague kindly gave us.
Date published: 2023-08-28
Rated 5 out of 5 by from Excellent customer feedback.Express delivery in a week period. Proper handling. Thank you.
Date published: 2022-07-28
Rated 4 out of 5 by from きれいサンプル使用しました。蛍光免疫染色で細胞の染色がきれいにできました。また使用を検討したいと思います。
Date published: 2021-08-30
Rated 3 out of 5 by from It workedI used this antibody for mice brain IHC. It worked but the signal was not so srong.
Date published: 2021-06-01
Rated 4 out of 5 by from Nice signal for Western blotThis antibody works nicely on Western blot samples from rat hippocampus. A clear double-band can be detected between 37 and 50 kDa, corresponding to the expected molecular weight of the protein. Antibody dilution: 1:500 in PBST with 5% nonfat dry milk.
Date published: 2021-02-17
Rated 4 out of 5 by from Für Kryoschnitte geeignetGute Färbeergebnisse, allerdings musste eine 1:20 Verdünnung eigesetzt werden. Klon E-5 brachte keine Ergebnisse.
Date published: 2020-10-15
Rated 4 out of 5 by from Immunohistochemistryで使用しました。マウスの脳切片を使用して、Immunohistochemistryで検出を行いました。感度よく検出できたと思います。
Date published: 2020-08-05
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_39
  • loc_ko_KR, sid_271390, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 15ms
  • REVIEWS, PRODUCT
Doublecortin/DCX 항체 (E-6) is rated 4.3 out of 5 by 39.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_39
  • loc_ko_KR, sid_271390, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 106ms
  • REVIEWS, PRODUCT