Doublecortin/DCX Antibody (E-6): sc-271390

Doublecortin Antibody (E-6) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect Doublecortin, a critical microtubule-associated protein involved in neuronal migration and brain development, across mouse, rat, and human species using applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Doublecortin antibody (E-6) targets an epitope encompassing amino acids 81-365 at the C-terminus of human Doublecortin, ensuring precise recognition and minimal cross-reactivity. Doublecortin, also called DC, DCX, DBCN, LISX, SCLH, and XLIS, plays a vital role in stabilizing microtubules, which is essential for proper migration of neurons during cerebral cortex development. By facilitating transport of neuronal precursors to appropriate locations, Doublecortin ensures formation of a functional and structurally sound brain. Mutations in the DCX gene lead to severe mental retardation and abnormal brain architecture, causing neurodevelopmental disorders such as lissencephaly and double cortex syndrome. Doublecortin maintains structural integrity of neuronal processes, crucial for synaptic plasticity and overall brain function. Doublecortin monoclonal antibody (E-6) is available in both non-conjugated form and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing researchers with versatile options for diverse experimental needs. Anti-Doublecortin antibody (E-6) supports advanced studies in neuroscience, developmental biology, and molecular mechanisms underlying neurological disorders, making this tool invaluable for understanding brain development and associated pathologies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Doublecortin/DCX Antibody (E-6) References:

1. A new activity of doublecortin in recognition of the phospho-FIGQY tyrosine in the cytoplasmic domain of neurofascin.  |  Kizhatil, K., et al. 2002. J Neurosci. 22: 7948-58. PMID: 12223548
2. Differential expression and cellular localization of doublecortin in the developing rat retina.  |  Lee, EJ., et al. 2003. Eur J Neurosci. 17: 1542-8. PMID: 12752371
3. Expression of doublecortin in tumours of the central and peripheral nervous system and in human non-neuronal tissues.  |  Bernreuther, C., et al. 2006. Acta Neuropathol. 111: 247-54. PMID: 16520969
4. Distinct roles of doublecortin modulating the microtubule cytoskeleton.  |  Moores, CA., et al. 2006. EMBO J. 25: 4448-57. PMID: 16957770
5. Doublecortin expression continues into adulthood in horizontal cells in the rat retina.  |  Wakabayashi, T., et al. 2008. Neurosci Lett. 442: 249-52. PMID: 18647639
6. Crystal Structures of the Human Doublecortin C- and N-terminal Domains in Complex with Specific Antibodies.  |  Burger, D., et al. 2016. J Biol Chem. 291: 16292-306. PMID: 27226599
7. Isolation of a Miller-Dieker lissencephaly gene containing G protein beta-subunit-like repeats.  |  Reiner, O., et al. 1993. Nature. 364: 717-21. PMID: 8355785
8. Folding of proteins with WD-repeats: comparison of six members of the WD-repeat superfamily to the G protein beta subunit.  |  Garcia-Higuera, I., et al. 1996. Biochemistry. 35: 13985-94. PMID: 8909296
9. Platelet-activating factor acetylhydrolase expression and activity suggest a link between neuronal migration and platelet-activating factor.  |  Albrecht, U., et al. 1996. Dev Biol. 180: 579-93. PMID: 8954729
10. A novel CNS gene required for neuronal migration and involved in X-linked subcortical laminar heterotopia and lissencephaly syndrome.  |  des Portes, V., et al. 1998. Cell. 92: 51-61. PMID: 9489699
11. Doublecortin, a brain-specific gene mutated in human X-linked lissencephaly and double cortex syndrome, encodes a putative signaling protein.  |  Gleeson, JG., et al. 1998. Cell. 92: 63-72. PMID: 9489700
12. LISsen up!  |  Walsh, CA. 1998. Nat Genet. 19: 307-8. PMID: 9697681

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Doublecortin/DCX Antibody (E-6) has 141 citations in a variety of scientific publications.

Primary antibodies like Anti-Doublecortin/DCX Antibody (E-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Doublecortin/DCX Antibody (E-6) Specifications

Alternate Product Name: Doublecortin

Product Clone Name: E-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 81-365

Epitope Target: Doublecortin

Epitope Species: human

Product Reactivity/Detection: Doublecortin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 141 citations in various scientific publications

Available Conjugates/Forms for Doublecortin/DCX Antibody (E-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Doublecortin/DCX antibody is Doublecortin/DCX Antibody (E-5): sc-390645.