DDX21 항체 (F-5)는 WB, IP, IF and ELISA으로 mouse유래의 DDX21 를 감지하는 데에 추천한다 .
IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-DDX21 항체 (F-5)를 제공한다.
WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for DDX21 Antibody (F-5) for WB applications. This reagent is now offered in a bundle with DDX21 Antibody (F-5) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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DEAD-box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome/spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX21 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 21), also known as GUA or GURDB, is a 783 amino acid protein that localizes to the nucleus and contains one helicase C-terminal domain and one helicase ATP-binding domain. Existing as multiple alternatively spliced isoforms, DDX21 functions as a component of the multi-protein B-WICH complex and acts as both a helicase that can unwind double-stranded RNA and as a foldase that can introduce secondary structures into single-stranded RNA. DDX21 exists as an autoantigen in people affected by watermelon stomach disease which is often characterized by chronic gastrointestinal bleeding.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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For Western Blot, is it recommended to use denatured or non-denatured conditions with DDX21 (F-5): sc-376758 monoclonal antibody?
Asked by: AbPolly
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.