DAT/Dopamine Transporter/SLC6A3 항체 (6-8D6) WB, IP, IF와 IHC(P)으로 mouse, rat와 human origin의 DAT을 검출할것을 권장합니다.

DAT/Dopamine Transporter/SLC6A3 항체 (6-8D6): sc-32259

DAT/Dopamine Transporter/SLC6A3 항체 (6-8D6) is rated 4.5 out of 5 by 11.
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데이터 시트

    • 안티-DAT/Dopamine Transporter/SLC6A3 항체 6-8D6 는 랫드 monoclonal IgG2a DAT/Dopamine Transporter/SLC6A3 항체, 10간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • raised against amino acids 180-218 corresponding to the second extracellular loop of DAT of human origin
    • 안티-DAT/Dopamine Transporter/SLC6A3 항체 (6-8D6)는 WB, IP, IF and IHC(P)으로 mouse, rat and human유래의 DAT 를 감지하는 데에 추천한다.
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-DAT/Dopamine Transporter/SLC6A3 항체 (6-8D6)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • DAT/Dopamine Transporter/SLC6A3 (6-8D6): sc-32259무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for DAT/Dopamine Transporter/SLC6A3 Antibody (6-8D6). This work is in progress.
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Are there reports using this DAT ab for Immunoprecipitation, if so which ones? Could it be used for magnetic isolation by coupling to Dynabeads?

Asked by: andresj1
Thank you for your question. DAT Antibody (6-8D6): sc-32259 has been tested and is recommended for IP applications with DAT of mouse, rat and human origin. As of now there are no publications in which this antibody has been used for immunoprecipitation.
Answered by: Tech Support Europe
Date published: 2020-07-14

Can I use it on rat sections, blocking with normal donkey serum?

Asked by: ann1
Thank you for your question. Yes, this antibody is mouse, rat and human reactive, so using it on rat samples is fine. If you are doing a Western blot we would recommend blocking in 5% milk with TBS-T. You can block in normal donkey serum if you are doing an IHC or IF, especially if you are using a donkey anti-rat secondary antibody. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-11-06

Is a blocking peptide for with this monoclonal antibody available?

Asked by: AbPolly
Thank you for your question. This DAT antibody sc-32259 is raised against a recombinant protein and not a short synthetic peptide and a blocking peptide is not possible to design for it.
Answered by: Technical Support 7
Date published: 2017-05-16
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Rated 5 out of 5 by from Works well for mouse brain tissue Purchased for IHC of mouse brain slices fixed in 0.4% PFA. Used 1:50 down to 1:4000 dilutions (in 4% BSA with 0.3% Triton-X100) and saw great staining of straitum.
Date published: 2021-07-15
Rated 4 out of 5 by from great antibody I acquired the antibody a while ago. Works greatly and as good as other DATmAb from other vendors. We chose to purchase after sampling the product.
Date published: 2019-03-22
Rated 3 out of 5 by from Good! I applied this antibody last year. This antibody couldn't use with rats. It's very sad.
Date published: 2018-03-02
Rated 5 out of 5 by from 功能很出色 上半年申请的该抗体,用于westernblot实验,样品为N2A细胞,上样量为50 ug,曝光结果较好。
Date published: 2017-09-27
Rated 5 out of 5 by from Clearness I used the antibody in WB at 1:300, PC12 cells. The protein band was clear, and there was no other bands. The pictures shows that the protein band is a little narrow, possibly because I used the 10% SDS separation gel.
Date published: 2017-09-18
Rated 5 out of 5 by from this Adrenergic binding of the beta - adrenergic receptor activates adenylate cyclase, whereas adrenergic binding of the α2 - adrenergic receptor inhibits adenylate cyclase. The action of the molecule can be excitatory or inhibitory, depending on the type of receptor to which it is bound.
Date published: 2017-02-08
Rated 5 out of 5 by from DAT (6-8D6) Antibody Works great for Western blot (WB), band at 80 kDa. We have not characterized the band above 90 kDa.
Date published: 2017-01-05
Rated 4 out of 5 by from Pretty good... Used in IPs and WB and it worked as expected. Stable for years when stored correctly.
Date published: 2016-12-05
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