DAP10 (h): 293T Lysate: sc-116977

The DAP10 (h): 293T Lysate is derived from the HEK293T cell line, a highly transfectable human embryonic kidney cell line frequently used in molecular biology research. This lysate contains DNAX-activating protein of 10 kDa (DAP10), an adaptor protein essential for the signaling of the NKG2D receptor, primarily found on natural killer (NK) cells and certain T-cell subsets. DAP10 facilitates the activation of these immune cells by linking NKG2D receptor engagement to downstream signaling cascades, including the PI3K-Akt pathway and the Grb2-Vav1 pathway. These pathways are critical for cellular activation, proliferation, and cytotoxic responses. The DAP10 (h): 293T Lysate is extensively utilized in research to study the molecular mechanisms of immune cell activation and the role of the NKG2D-DAP10 axis in immune surveillance. It serves as a valuable tool for in vitro assays to investigate protein-protein interactions, phosphorylation events, and the regulatory processes modulating DAP10 function. Researchers employ this lysate to screen for potential activators or inhibitors of DAP10-mediated signaling, advancing the understanding of how DAP10 influences immune cell behavior and its broader implications in immune system regulation. This lysate thus plays a crucial role in elucidating the signaling networks that govern immune responses.