Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CUG-BP2 CRISPR/Cas9 KO Plasmid (h): sc-403267
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CUG-BP2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CUG-BP2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CUG-BP2 Antibody (1H2): sc-47731
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CUG-BP2 CRISPR/Cas9 KO Plasmid (h) | sc-403267 | 20 µg | $397.00 |
Overview
CELF2 (CUG-BP2) is an RNA-binding protein of the CELF family that coordinates post-transcriptional gene regulation by controlling alternative splicing, mRNA stability, and translation across diverse transcripts. It participates in coupling RNA processing to signaling inputs during cell differentiation and stress responses, influencing networks involved in cytoskeletal organization, cell-cycle control, and apoptosis. CELF2-dependent RNA programs contribute to tissue-specific transcript isoform selection and are frequently studied in the context of immune cell activation and developmental gene regulation. Dysregulated CELF2 activity has been associated with altered splicing landscapes observed in neuromuscular and cancer-related models, motivating mechanistic studies of its RNA targets and regulatory partners.
CUG-BP2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CELF2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CELF2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CELF2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CUG-BP2 protein expression.
This CRISPR knockout system enables efficient generation of CELF2-deficient cell models for investigation of CUG-BP2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CELF2 exon(s) critical for CUG-BP2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CELF2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CUG-BP2 CRISPR/Cas9 KO Plasmid (h) and CUG-BP2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CELF2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CUG-BP2 HDR Plasmid (h) and CUG-BP2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CELF2 homology arms to support homology-directed repair at defined CELF2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.