CstF-64T CRISPR/Cas9 KO Plasmid (h): sc-406358
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CstF-64T CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CstF-64T genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CstF-64T CRISPR/Cas9 KO Plasmid (h) | sc-406358 | 20 µg | $397.00 |
Overview
CSTF2T encodes CstF-64T, an RNA-binding subunit of the cleavage stimulation factor complex that directs 3′ end processing and polyadenylation of pre-mRNAs. By influencing cleavage site choice and alternative polyadenylation, CstF-64T contributes to transcript isoform diversity, mRNA stability, and translational control, with downstream effects on cell state and differentiation programs. Its activity is particularly relevant in germline contexts and in models where shifts in 3′ UTR length remodel post-transcriptional regulation. Dysregulation of cleavage and polyadenylation machinery, including CSTF2T-linked pathways, is associated with broad gene expression changes observed in proliferative and stress-adaptation phenotypes studied in cancer and developmental biology.
CstF-64T CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CSTF2T gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CSTF2T together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CSTF2T open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CstF-64T protein expression.
This CRISPR knockout system enables efficient generation of CSTF2T-deficient cell models for investigation of CstF-64T signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CSTF2T exon(s) critical for CstF-64T function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CSTF2T genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CstF-64T CRISPR/Cas9 KO Plasmid (h) and CstF-64T CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CSTF2T locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CstF-64T HDR Plasmid (h) and CstF-64T HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CSTF2T homology arms to support homology-directed repair at defined CSTF2T target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.