CRTAP Antibody (L-24): sc-100920

CRTAP Antibody (L-24) is a mouse monoclonal IgG2a antibody that detects CRTAP (cartilage associated protein) in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). CRTAP is a secreted protein primarily located in the extracellular space, where CRTAP plays a crucial role in collagen post-translational modifications, extracellular fibril assembly, and intracellular trafficking. CRTAP is predominantly expressed in articular chondrocytes and is essential for normal bone formation, as CRTAP contains a signal peptide and a tetratricopeptide-like helical domain. Within the endoplasmic reticulum, CRTAP forms a complex with Gros1 and cyclophilin B, which is vital for the efficient 3-hydroxylation of prolyl residues in Collagen Type I molecules, the major structural proteins of skin and bone. Proper functioning of CRTAP is critical, as mutations in the gene encoding CRTAP can lead to autosomal recessive osteogenesis imperfecta (OI) types 7 and 2B, conditions characterized by bone fragility and a heightened risk of fractures. OI type 7 is a milder form of the disorder, while OI type 2B is associated with neonatal lethality, underscoring the importance of CRTAP in skeletal health and development.

The preferred CRTAP antibody is CRTAP Antibody (E-1): sc-393136.