CRBP I Antibody (F3): sc-53989

CRBP I Antibody (F3) is a mouse monoclonal IgG1 antibody that detects cellular retinol-binding protein I (CRBP I) in mouse, rat, human, and bovine samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-CRBP I antibody (F3) recognizes a protein crucial for cellular uptake and transport of retinol, the alcoholic form of vitamin A, which is vital for numerous biological processes including cell growth, differentiation, and vision. CRBP I (F3) monoclonal antibody targets a protein primarily located in the cytoplasm, characterized by a unique structure composed of ten antiparallel β-strands forming a β-barrel, encapsulating the retinol molecule, along with two α-helices that seal the ends of the barrel. This structural configuration allows CRBP I to solubilize and detoxify retinol, facilitating transport within the cytoplasm and ensuring availability for conversion into retinoic acid, the active form of vitamin A. CRBP I is expressed in various human tissues, including the ovary, adrenal and pituitary glands, and testis, with expression being modulated by transforming growth factor beta (TGFβ). CRBP I′s interaction with retinol and derivatives underscores an importance in maintaining vitamin A homeostasis and supporting essential physiological functions.

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CRBP I Antibody (F3) References:

1. Aberrant cellular retinol binding protein 1 (CRBP1) gene expression and promoter methylation in prostate cancer.  |  Jerónimo, C., et al. 2004. J Clin Pathol. 57: 872-6. PMID: 15280411
2. Cellular retinol-binding protein type I (CRBP-I) regulates adipogenesis.  |  Zizola, CF., et al. 2010. Mol Cell Biol. 30: 3412-20. PMID: 20498279
3. Cellular retinol-binding protein 1 (CRBP-1) regulates osteogenenesis and adipogenesis of mesenchymal stem cells through inhibiting RXRα-induced β-catenin degradation.  |  Xu, L., et al. 2012. Int J Biochem Cell Biol. 44: 612-9. PMID: 22230368
4. The Effect of Metformin Treatment on CRBP-I Level and Cancer Development in the Liver of HBx Transgenic Mice.  |  Kim, JH., et al. 2013. Korean J Physiol Pharmacol. 17: 455-61. PMID: 24227948
5. CRBP-1 expression in ovarian cancer: a potential therapeutic target.  |  Doldo, E., et al. 2014. Anticancer Res. 34: 3303-12. PMID: 24982334
6. Ligand Binding Induces Conformational Changes in Human Cellular Retinol-binding Protein 1 (CRBP1) Revealed by Atomic Resolution Crystal Structures.  |  Silvaroli, JA., et al. 2016. J Biol Chem. 291: 8528-40. PMID: 26900151
7. Structural and molecular determinants affecting the interaction of retinol with human CRBP1.  |  Menozzi, I., et al. 2017. J Struct Biol. 197: 330-339. PMID: 28057518
8. CRBP-1 over-expression is associated with poor prognosis in tongue squamous cell carcinoma.  |  Chen, Y., et al. 2018. BMC Cancer. 18: 514. PMID: 29720147
9. Discovery of Nonretinoid Inhibitors of CRBP1: Structural and Dynamic Insights for Ligand-Binding Mechanisms.  |  Plau, J., et al. 2023. ACS Chem Biol. 18: 2309-2323. PMID: 37713257
10. Gene expression of cellular retinol-binding protein I (CRBP I) is affected by dietary proteins in the rat liver.  |  Kato, S., et al. 1993. J Nutr Sci Vitaminol (Tokyo). 39: 545-54. PMID: 7516425

The preferred CRBP I antibody is CRBP I Antibody (B-8): sc-271208.