Che-1 Antibody (AATF2B6): sc-81225

Che-1 Antibody (AATF2B6) is a mouse monoclonal IgG1 antibody that detects Che-1 protein of human origin by western blotting (WB) and immunoprecipitation (IP). Anti-Che-1 antibody (AATF2B6) is available as a non-conjugated format. Che-1, also known as apoptosis-antagonizing transcription factor (AATF), is a crucial nuclear protein that plays a significant role in regulating cell cycle progression and apoptosis. Che-1 becomes hyperphosphorylated during G1/S phase transition, which is essential for proper cell cycle progression. Che-1 functions as a general inhibitor of histone deacetylase HDAC1, and Che-1 binding to retinoblastoma protein (Rb) pocket region can displace HDAC1 from Rb/E2F complexes, thereby activating E2F target genes that promote cell cycle progression. Che-1 has been shown to antagonize pro-apoptotic effects of PAR4 (prostate apoptosis response 4), a protein associated with neuronal degeneration in Alzheimer′s disease. Che-1 interaction with PAR4 suggests a potential regulatory role in modulating PAR4 activity, which is critical for understanding implications in neurodegenerative diseases. Che-1 co-localizes with PAR4 in both cytoplasmic and nuclear compartments, highlighting importance in cellular signaling pathways.

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Che-1 Antibody (AATF2B6) References:

1. Identification of novel transcription factor-like gene from human intestinal cells.  |  Lindfors, K., et al. 2000. Biochem Biophys Res Commun. 276: 660-6. PMID: 11027528
2. Che-1 arrests human colon carcinoma cell proliferation by displacing HDAC1 from the p21WAF1/CIP1 promoter.  |  Di Padova, M., et al. 2003. J Biol Chem. 278: 36496-504. PMID: 12847090
3. AATF inhibits aberrant production of amyloid beta peptide 1-42 by interacting directly with Par-4.  |  Guo, Q. and Xie, J. 2004. J Biol Chem. 279: 4596-603. PMID: 14627703
4. TSG101 interacts with apoptosis-antagonizing transcription factor and enhances androgen receptor-mediated transcription by promoting its monoubiquitination.  |  Burgdorf, S., et al. 2004. J Biol Chem. 279: 17524-34. PMID: 14761944
5. AATF protects neural cells against oxidative damage induced by amyloid beta-peptide.  |  Xie, J. and Guo, Q. 2004. Neurobiol Dis. 16: 150-7. PMID: 15207272
6. Phosphoproteome analysis of the human mitotic spindle.  |  Nousiainen, M., et al. 2006. Proc Natl Acad Sci U S A. 103: 5391-6. PMID: 16565220
7. Functional characterization of AATF transcriptome in human leukemic cells.  |  Kaul, D. and Mehrotra, A. 2007. Mol Cell Biochem. 297: 215-20. PMID: 17006618