CDR2 항체 (W-12) WB, IP, IF 와ELISA으로 human origin의 CDR2을 검출할것을 권장합니다.

CDR2 항체 (W-12): sc-100320

CDR2 항체 (W-12) is rated 5.0 out of 5 by 3.
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Datasheets

    • 안티-CDR2 항체 W-12 는 마우스 monoclonal IgG2a (kappa light chain) CDR2 항체 이며 100 µg/ml으로 제공합니다.
    • human origin의 CDR2의 전체인 296-405 아미노산을 항원으로 사용하였습니다.
    • WB, IP, IF 와ELISA으로 human origin의 CDR2을 검출할것을 권장합니다.
    • 강력추천하는 conventional secondary anti-mouse IgG reagents의 대체제품 보기m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP).
    • CDR2 (W-12): sc-100320무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.

I am using CDR2 (W-12): sc-100320 for immunofluorescence, what dilution of this antibody should I use?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from this antibody works for my experiment! The molecular wight might a little bit different to the image shown on the website. its about 63kDa in both HFFF and HEK cells. Band was clear in WB, haven't try IF or IP yet.
Date published: 2018-01-18
Rated 5 out of 5 by from Beautiful immunofluorescence nuclear staining Beautiful immunofluorescence nuclear staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-04-27
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