Date published: 2025-9-6
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CUTL1/CUX1/CDP Antibody (B-10) - Western Blotting - Image 314151
CDP 항체(B-10): sc-514008. BJAB (A) 및 K-562 (B) 핵 추출물에서 CDP 발현의 웨스턴 블롯 분석.
CDP 항체(B-10): sc-514008. BJAB (A) 및 K-562 (B) 핵 추출물에서 CDP 발현의 웨스턴 블롯 분석.

CUTL1/CUX1/CDP 항체 (B-10): sc-514008

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데이터 시트
  • CUTL1/CUX1/CDP 항체 B-10 는 마우스 monoclonal IgG3 κ CUTL1/CUX1/CDP 항체, 22간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 항원결정부는 mouse origin의 CDP의 내부에 위치한 1308-1332의 아미노산에 해당됩니다.
  • WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat와 human origin의 CDP을 검출할것을 권장합니다.
  • IP에 사용가능한 agarose결합제품; HRP/b> for WB, IHC(P) and ELISA; IF, IHC(P) 와 FCM에 사용가능한 phycoerythrin/b>결합제품, FITC결합제품, Alexa Fluor® 488/b>결합제품 와 Alexa Fluor® 647/b> 결합제품 이 있습니다.
  • ChIP 에 사용하는 TransCruz시약(sc-514008 X, 200 µg/0.1 ml)이 있습니다.
  • CUTL1/CUX1/CDP (B-10): sc-514008의 무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG3 BP-HRP 는CUTL1/CUX1/CDP 抗体 (B-10) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은CUTL1/CUX1/CDP 항체(B-10) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다).

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CDP 항체(B-10)는 마우스, 쥐 및 인간 유래의 CDP 단백질을 WB, IP, IF, IHC(P) 및 ELISA로 검출하는 IgG3 κ 마우스 모노클로날 CDP 항체(CUX1 항체 또는 CUTL1 항체로도 지정됨)입니다. CDP 항체(B-10)는 비접합 항-CDP 항체 형태뿐만 아니라 아가로스, HRP, PE, FITC 및 다중 Alexa Fluor® 접합체를 포함한 여러 접합 형태의 항-CDP 항체 형태로도 사용할 수 있습니다. CDP(CCAAT 치환 단백질용)는 발달 조절 유전자의 전사를 억제하는 것으로 확인되었습니다. 이 단백질은 다양한 유전자의 프로모터 영역에 결합하기 위해 전사 활성화 단백질과 경쟁하는 것으로 보이는 호모도메인 단백질입니다. CDP에는 DNA 결합 도메인으로 기능하는 세 개의 절단 반복이 포함되어 있습니다. 절단 반복 도메인은 호모도메인 DNA 결합과 함께 또는 독립적으로 DNA에 결합할 수 있는 능력이 있음이 입증되었습니다. CDP는 CDP 외에도 사이클린 A, Cdc2 및 Rb 관련 단백질을 포함하는 HiNF-D 복합체의 DNA 결합 서브유닛인 것으로 밝혀졌습니다. 히스톤 발현은 세포 주기에서 S기로의 전환에 필요합니다. HiNF-D 복합체는 히스톤 H4, H3 및 H1 유전자의 전사를 조절하여 세포가 G1 단계에서 S 단계로 진행하도록 합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

CUTL1/CUX1/CDP 항체 (B-10) 참고문헌:

  1. 인간 CCAAT 치환 단백질은 초파리 동종 단백질인 절단과 상동합니다.  |  Neufeld, EJ., et al. 1992. Nat Genet. 1: 50-5. PMID: 1301999
  2. DREF와 Cut에 의한 초파리 PCNA 유전자 프로모터의 길항적 조절.  |  Seto, H., et al. 2006. Genes Cells. 11: 499-512. PMID: 16629902
  3. 분화에 의한 Cutl1/CDP의 절단은 유방 유전자 발현을 조절하는 새로운 우성-음성 이소폼을 생성합니다.  |  Maitra, U., et al. 2006. Mol Cell Biol. 26: 7466-78. PMID: 17015474
  4. p75 CCAAT 치환 단백질/컷 호모박스 이소폼을 발현하는 형질전환 마우스는 골수 증식성 질환과 유사한 골수성 백혈병에 걸립니다.  |  Cadieux, C., et al. 2006. Cancer Res. 66: 9492-501. PMID: 17018605
  5. 인간 컷 호모도메인 단백질에서 보존된 컷 반복은 DNA 결합 도메인으로 기능합니다.  |  Harada, R., et al. 1994. J Biol Chem. 269: 2062-7. PMID: 7904999
  6. 마우스 홈도메인 단백질 Phox2는 Cux/CDP와 함께 Ncam 프로모터 활동을 조절하며 신경전달물질 표현형의 추정 결정인자입니다.  |  Valarché, I., et al. 1993. Development. 119: 881-96. PMID: 7910552
  7. CCAAT 치환 단백질은 여러 전사 활성화제와 경쟁하여 근위부 gp91phox 프로모터의 네 부위에 결합합니다.  |  Luo, W. and Skalnik, DG. 1996. J Biol Chem. 271: 18203-10. PMID: 8663528
  8. CDP/cut은 히스톤 유전자 전사인자 HiNF-D의 DNA 결합 하위 단위로, 전사인자 E2F와 무관하게 G1/S 단계 세포 주기 전환점에서 유전자를 조절하는 메커니즘입니다.  |  van Wijnen, AJ., et al. 1996. Proc Natl Acad Sci U S A. 93: 11516-21. PMID: 8876167

주문정보

제품명카탈로그 번호 단위가격수량관심품목

CUTL1/CUX1/CDP 항체 (B-10)

sc-514008
200 µg/ml
$316.00

CUTL1/CUX1/CDP (B-10): m-IgG3 BP-HRP 번들

sc-550607
200 µg Ab; 40 µg BP
$354.00

CUTL1/CUX1/CDP 항체 (B-10) AC

sc-514008 AC
500 µg/ml, 25% agarose
$416.00

CUTL1/CUX1/CDP 항체 (B-10) Alexa Fluor® 488

sc-514008 AF488
200 µg/ml
$357.00

CUTL1/CUX1/CDP 항체 (B-10) Alexa Fluor® 647

sc-514008 AF647
200 µg/ml
$357.00

CUTL1/CUX1/CDP 항체 (B-10) FITC

sc-514008 FITC
200 µg/ml
$330.00

CUTL1/CUX1/CDP 항체 (B-10) HRP

sc-514008 HRP
200 µg/ml
$316.00

CUTL1/CUX1/CDP (B-10) 중화펩타이드

sc-514008 P
100 µg/0.5 ml
$68.00

CUTL1/CUX1/CDP 항체 (B-10) PE

sc-514008 PE
200 µg/ml
$343.00

CUTL1/CUX1/CDP 항체 (B-10) X

sc-514008 X
200 µg/0.1 ml
$316.00

Hi, I used this antibody for my whole mount brain imaging with the technique called iDISCO, and I saw good staining. I saw the signal of Cux1 is mainly in the cytosol instead of the nucleus, which is suggested by the literatures. 

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext 1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-08-11

Hello, I am interested in ordering this antibody for IP. I have some literature citing old polyclonal Ab for IP with great success but the reviews on this monoclonal are worrisome. Also, I am interested in using magnetic beads for labeling. Please advice.

Asked by: sbagla
Thank you for your question. This antibody has been established for IP applications in samples of mouse, rat and human origin in our labs and offer our warranty for this use. If you purchase the antibody and it does not perform as expected in the recommended species and application, please contact our Technical Service Department. If we are unable to help you troubleshoot the antibody, we can provide you with a complimentary replacement antibody, credit or refund. Our warranty is for one year from the date of purchase.
Answered by: Tech Support Europe
Date published: 2019-06-12

Hi there! I've been looking at the wonderful IHC images obtained with the CDP M-222 antibody; however, I'm concerned with the bad reviews of its replacement. I want to use CDP B-10 for PFA fixed free floating mouse brain sections.

Asked by: JuanSA
Thank you for your question. We have not tested this antibody in free floating mouse brain tissue sections. However, we have had excellent results using the antibody in formalin-fixed paraffin-embedded human thyroid and upper stomach tissues. Unlike in some of the customer product reviews, we used the primary antibody at a dilution of 1:50. We do warranty the antibody to work for mouse tissues. If you purchase the antibody and it does not perform as expected in the recommended species and application, please contact our Technical Service Department. If we are unable to help you troubleshoot the antibody, we can provide you with a complimentary replacement antibody, credit or refund. Our warranty is for one year from the date of purchase.
Answered by: Technical Support
Date published: 2018-11-21

What application is the blocking peptide sc-514008 P appropriate for?

Asked by: jenniferc
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 7
Date published: 2017-01-22
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Rated 5 out of 5 by from Good antibodyThe antibody arrived quickly. It works well for immunostaining
Date published: 2025-07-07
Rated 1 out of 5 by from No signal at allWe tested this antibody on mouse embryonic brain tissue to stain neocortical upper layers. No signal at all with or without antigen retrieval. It's a shame that M222 antibody was replaced by this one.
Date published: 2020-03-04
Rated 1 out of 5 by from Didn't WorkOur lab has previously used and gotten great results from the SCBT's discontinued polyclonal CDP ab. We purchased the monoclonal since the poly was no longer available, but it did not work. Despite multiple attempts with and without antigen retrieval from two different lot numbers of the antibody, we got no results. SCBT has, however, been extremely helpful in terms of providing a second aliquot of no charge. They responded quickly and we are very happy with their customer service.
Date published: 2019-04-16
Rated 1 out of 5 by from Did not work for mouse brain IHCWe tested this antibody in free floating sections of perfused mouse brain. No specific signal was observed at a 1:100 dilution. Antigen retrieval with citrate buffer pH 6.0 at 85°C for 25 min also failed to produce any specific staining.
Date published: 2018-11-15
Rated 1 out of 5 by from No bands were observed in WBUsed 1:100 dilution for mouse tissue (Liver, brain) as well as AML12 hepatocyte cell line nuclear and cytoplasmic proteins for 1 overnight incubation in 5% milk TBS-T blocking buffer. No bands whatsoever was observed. Actin and Nucleolin band as loading control was very good, so it was not the protein problem. As well, same secondary Ab was used for the Actin that was used with this Ab, so it was not the secondary antibody issue either.
Date published: 2018-03-20
Rated 1 out of 5 by from That's too bad..I got the sample of this and tried. No matter how I tried to stain with this antibody, there's only very blurred signal on the sections
Date published: 2018-02-26
Rated 1 out of 5 by from Did not work for IFTried it for IF on mouse brain slices (1:500) and it did not work
Date published: 2018-01-30
Rated 5 out of 5 by from niceGreat antibody！1：1000 used in the u251 cells by Western Blotting, I got satisfactory results.
Date published: 2017-11-03
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CUTL1/CUX1/CDP 항체 (B-10) is rated 2.7 out of 5 by 19.
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