CBP/KAT3A/CREBBP 항체 (C-1) recommended for detection of by WB, IP, IF, IHC(P)와 ELISA으로 mouse, rat, human와 origin의 CBP p265을 검출할것을 권장합니다.

CBP/KAT3A/CREBBP 항체 (C-1): sc-7300

CBP/KAT3A/CREBBP 항체 (C-1) is rated 4.3 out of 5 by 37.
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데이터 시트

    • 안티-CBP/KAT3A/CREBBP 항체 C-1 는 마우스 monoclonal IgG1 κ CBP/KAT3A/CREBBP 항체, 120간행물에 인용, 이며 200 µg/ml으로 제공합니다.
    • specific for an epitope mapping between amino acids 2423-2442 at the C-terminus of CBP of human origin (identical to corresponding mouse sequence)
    • CBP/KAT3A/CREBBP 항체 (C-1)는 WB, IP, IF, IHC(P) and ELISA으로mouse, rat, human and 유래의 CBP p265 를 감지하는 데에 추천한다 ; 이외에, canine, bovine, porcine and avian등 species와 반응할수 있습니다
    • IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-CBP/KAT3A/CREBBP 항체 (C-1)를 제공한다.
    • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
    • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
    • Gel Supershift와 ChIP 에 사용하는 TransCruz시약(sc-7300 X, 200 µg/0.1 ml)이 있습니다.
    • CBP/KAT3A/CREBBP (C-1): sc-7300무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.

Do you have a directly conjugated isotype control for this antibody. I bought sc-7300 AF594 and need a proper control to show that the positive signal is not a non-specific binding

Asked by: zhangll
Thank you for your question. The appropriate isotype control for AF594-conjugated sc-7300 is normal mouse IgG1 Alexa Fluor® 594: sc-516620.
Answered by: Tech Support Europe
Date published: 2020-09-07

I recently purchased this antibody. However, I need to know if this antibody cross-reacts with the closely related p300 protein (KAT3B aka EP300) in human cells. A rapid and accurate response would be greatly appreciated. Thanks, Isaac

Asked by: IHilton
Thank you for your question. This mouse monoclonal antibody, CBP (C-1): sc-7300, is specific for CBP (CREB-binding protein). We have not specifically tested this clone for potential cross-reactivity with KAT3B aka EP300, however this antibody has been validated for specificity using our human siRNA for knockdown against the human gene target, CBP siRNA (h): sc-29244.
Answered by: Technical Support
Date published: 2019-06-24

antibody Cat#sc-7300: how do I get in possession of the 10ug sample as advertised on your website? Thanks

Asked by: Seia
Thank you for your question. For a limited time, we are offering a free 10 µg sample of our mouse monoclonal antibody, CBP Antibody (C-1): sc-7300. Please contact our Technical Service Department and we would be happy to arrange a complimentary shipment for you. You may call us toll free at (800) 457-3801 option 2, or email [email protected], or use our live chat!
Answered by: Technical Support
Date published: 2017-01-25

Is there any data showing this antibody is specific to CBP?

Asked by: jenni
Thank you for your question. We have immunofluorescence data with methanol fixed HeLa cells treated with siRNA to show knockdown of signal, confirming specificity to CBP.
Answered by: Technical Support 13
Date published: 2020-08-10
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Rated 5 out of 5 by from Looks visible and great This antibody works well with transfer buffer that included 0.1%SDS and only 10% methanol. Use for incubation at 1:500
Date published: 2018-12-12
Rated 5 out of 5 by from Works in ChIP analysis In ChIP analysis, tThis antibody could detect forskolin-dependent binding of CBP to c-Fos promoter in HEK293FT cells.
Date published: 2018-10-29
Rated 1 out of 5 by from not working for me in IFA I was unfortunately not successful in getting a signal with 96 well plate probed with recombinant CBP tagged protein O/N at 4C using a fluorescent secondary antibody for 1hr. Antibodies were concentrated as high as 1:50 in PBS-BSA and incubated for 1-2hrs at RT. I used an anti-CBP from a different vendor that gave me a specific signal. This was also tried on fixed cell lines expressing CBP on the surface with negative results as well.
Date published: 2018-10-24
Rated 5 out of 5 by from Good Product 2 The antibody worked well at 1:500 dilution using mouse brain tissue extract.
Date published: 2018-08-29
Rated 2 out of 5 by from なんとも言えない 過剰発現したものには当たりましたが、内在性のものはdetectできませんでした。当たりが弱い気はしました。
Date published: 2018-07-03
Rated 2 out of 5 by from not satisfied for IF I tested this AB in IF (range 1/20 to 1/100) both in cells and brain E16 mouse the signal was really low (PFA fixation in both case)
Date published: 2018-03-22
Rated 2 out of 5 by from no good signal in IF I didn't detect good staining signal in 4%PFA fixed embyronic mouse brain(after antigen retrieval)
Date published: 2018-03-07
Rated 3 out of 5 by from It works for western blotting. It detected a faint band with the expected size in nuclear extracts of immortalized HFFs, but not in total protein extracts (1:500 in 5% milk in TBS-0.1% Tween overnight at 4C).
Date published: 2018-02-23
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