C19orf12 CRISPR/Cas9 KO Plasmid (h): sc-406640
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C19orf12 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the C19orf12 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C19orf12 CRISPR/Cas9 KO Plasmid (h) | sc-406640 | 20 µg | $397.00 |
Overview
C19orf12 encodes a small, mitochondria-associated protein implicated in lipid handling and maintenance of mitochondrial homeostasis, with links to cellular responses to oxidative stress and iron metabolism. Functional studies connect C19orf12 to mitochondrial membrane biology and processes that influence energy production, redox balance, and organelle quality control pathways such as mitophagy. Disruption of C19orf12 is associated with neurodegeneration with brain iron accumulation (NBIA), highlighting its relevance to mechanisms of neuronal vulnerability, iron dysregulation, and mitochondrial dysfunction. As a result, C19orf12 is frequently studied in models that interrogate mitochondrial stress signaling, lipid remodeling, and neurodegenerative disease pathways.
C19orf12 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the C19orf12 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the C19orf12 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the C19orf12 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish C19orf12 protein expression.
This CRISPR knockout system enables efficient generation of C19orf12-deficient cell models for investigation of C19orf12 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting C19orf12 exon(s) critical for C19orf12 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple C19orf12 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by C19orf12 CRISPR/Cas9 KO Plasmid (h) and C19orf12 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the C19orf12 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by C19orf12 HDR Plasmid (h) and C19orf12 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by C19orf12 homology arms to support homology-directed repair at defined C19orf12 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.