C15orf27 Double Nickase Plasmid (h): sc-414201-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- C15orf27 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- C15orf27 Double Nickase Plasmid (h) and C15orf27 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TMEM266. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
C15orf27 Double Nickase Plasmid (h) | sc-414201-NIC | 20 µg | $410.00 |
TMEM266 (also annotated as C15orf27) encodes a predicted multi-pass transmembrane protein with limited functional characterization in human cells. Expression patterns and sequence features suggest a role in membrane-associated processes such as protein trafficking, organelle communication, or regulation of receptor-linked signaling, with potential impacts on cellular homeostasis. As an incompletely annotated gene, TMEM266 is frequently prioritized in transcriptomic and functional genomics studies to clarify pathway connectivity and context-specific cellular phenotypes. Dysregulated expression of uncharacterized transmembrane proteins can serve as a molecular readout of altered signaling states in disease-relevant models, supporting mechanistic research rather than clinical interpretation.
C15orf27 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TMEM266 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TMEM266. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TMEM266 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TMEM266-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.