아미노 산 351-400 within a C-terminal cytoplasmic domain of β3-AR of mouse origin 간의 피토프 매핑 에 특이적인다
안티-beta 3 Adrenergic Receptor/ADRB3/β3-AR 항체 (C-5)는 WB, IP, IF and ELISA으로 mouse and rat유래의 β3-AR 를 감지하는 데에 추천한다.
IP를 위해 agarose ;WB, IHC(P) and ELISA를 위해 HRP ;또는 IF, IHC(P) and FCM를 위해 phycoerythrin or FITC 에 결합된 Anti-beta 3 Adrenergic Receptor/ADRB3/β3-AR 항체 (C-5)를 제공한다.
WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for beta 3 Adrenergic Receptor/ADRB3/β3-AR Antibody (C-5) for WB applications. These reagents are now offered in bundles with beta 3 Adrenergic Receptor/ADRB3/β3-AR Antibody (C-5) (see ordering information below).
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β3-AR Antibody (C-5) is a high quality monoclonal beta 3-AR antibody (also designated ADRB3 antibody, Adrenoceptor Beta 3 antibody, Adrenergic Beta-3- Receptor antibody, Beta-3 Adrenoreceptor antibody, Beta-3 Adrenoceptor antibody, BETA3AR antibody, or B3AR antibody) suitable for the detection of the beta 3-AR protein of mouse and rat origin. β3-AR Antibody (C-5) is available as both the non-conjugated anti-beta 3-AR antibody form, as well as multiple conjugated forms of anti-beta 3-AR antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Beta 3-adrenergic receptors (b3-ARs) bind cathecholamines (epinephrine, norepinephrine), and primarily regulate lipolysis and thermogenesis in adipose. b3-ARs are present in adipose tissues, heart, and in smooth muscle of bladder, colon, small intestine, and stomach. The human corpus cavernosum exhibits basal b3-AR-mediated vasorelaxant tone and activity is linked to inhibition of the RhoA/Rho-kinase pathway. b3-AR interacts directly with the SH3 domain of Src through proline-rich motifs (PXXP) in the third intracellular loop and the carboxy-terminus.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What blocking solution should I use with β3-AR (C-5): sc-515763 monoclonal antibody for Western blot?
Asked by: Germaine
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: