Date published: 2025-9-7

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BAP1 항체 (C-4): sc-28383

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데이터 시트
  • BAP1 항체 C-4 는 마우스 monoclonal IgG1 κ BAP1 항체, 337간행물에 인용, 이며 200 µg/ml으로 제공합니다.
  • 안티-BAP1 항체 (C-4)는 WB, IP, IF, IHC(P) and ELISA으로 mouse, rat and human유래의 BAP 1 를 감지하는 데에 추천한다.
  • BAP1 항체(C-4)는 IP용으로 agarose에 결합된 형태, WB, IHC(P), ELISA용으로 HRP에 결합된 형태, IF, IHC(P), FCM용으로 phycoerythrin 또는 FITC에 결합된 형태로 제공됩니다.
  • WB (RGB), IF, IHC(P) 와FCM, RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647결합제품도 있습니다.
  • WB (NIR), IF와FCM,Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems에 사용가능한 Alexa Fluor® 680 or Alexa Fluor® 790 결합제품도 있습니다.
  • BAP1 (C-4): sc-28383무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 는 BAP1 항체 (C-4) 가 WB and IHC(P) 에 응용될 때 우선으로 사용하는 이차 항체 검사 시약입니다.이 시약은BAP1 항체(C-4) 와 세트로 제공드립니다. (아래의 주문 정보를 참고해 주시길 바랍니다).

빠른 링크

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BAP1 항체(C-4)는 마우스, 랫트, 인간에서 유래된 BAP1 단백질을 웨스턴 블롯(WB), 면역침전법(IP), 면역형광법(IF), 면역조직화학, 효소결합면역흡착분석법(ELISA)을 통해 검출하는 마우스 단일클론 IgG1 카파 경쇄 항체입니다. BAP1 항체(C-4)는 비접합형과 아가로스, 과산화효소(HRP), 피코에리트린(PE), 플루오레세인 이소티오시아네이트(FITC), 다중 알렉사 플루오르® 접합체 등 다양한 접합체 형태로 제공됩니다. BRCA1-associated protein 1로도 알려진 BAP1은 단백질 분해 조절과 세포 항상성 유지에 필수적인 유비퀴틴 가수분해 효소로서 기능합니다. 항-BAP1 항체(C-4)는 BRCA1에 의한 세포 성장 억제를 강화하여 암 예방에 기여합니다. 염색체 17q에 위치한 BRCA1 유전자의 돌연변이는 유방암과 난소암의 조기 발병률이 높은 가족의 약 45%에서 발견됩니다. BAP1은 729개의 아미노산으로 구성되어 있으며, 종양 억제에 관여하는 BARD1과 같은 다른 단백질과 상호 작용합니다. BAP1(C-4) 항체가 BRCA1에 결합하고 중요한 세포 경로에 관여하기 때문에 이 제품은 암 연구 및 치료제 개발에 유용합니다.

연구용으로만 사용가능합니다. 진단이나 치료용으로 사용불가합니다.

Alexa Fluor®는 미국 오리건주 Molecular Probes Inc.의 상표입니다.

LI-COR®와 Odyssey®는 LI-COR Biosciences의 등록 상표입니다.

BAP1 항체 (C-4) 참고문헌:

  1. 염색체 17q12-q23의 가족성 유방암-난소암 유전자좌.  |  Narod, SA., et al. 1991. Lancet. 338: 82-3. PMID: 1676470
  2. 조기 발병 가족성 유방암과 염색체 17q21의 연관성.  |  Hall, JM., et al. 1990. Science. 250: 1684-9. PMID: 2270482
  3. BAP1의 약리학적 억제는 HERC2를 모집하여 BRCA1-BARD1을 경쟁적으로 해리시키고, DNA 복구를 억제하며, CRC를 방사선 요법에 민감하게 만듭니다.  |  Yue, X., et al. 2023. Acta Pharm Sin B. 13: 3382-3399. PMID: 37655321
  4. BAP1 비활성화 흑색종 세포의 전형적인 조직학 너머.  |  Donati, M. and Kazakov, DV. 2024. Pathol Res Pract. 259: 155162. PMID: 38326181
  5. ATF2/BAP1 축은 P53 경로를 통해 지주막하 출혈 후 신경 세포 사멸을 매개합니다.  |  Tian, Q., et al. 2024. Stroke. 55: 2113-2125. PMID: 38965653
  6. 종양 억제제 BAP1은 SLC7A11과 NADPH 레벨의 조절을 통해 이황화합물 축적을 억제합니다.  |  Wang, J., et al. 2024. Oncogenesis. 13: 31. PMID: 39266549
  7. 원발성 유방암 및 난소암의 BRCA1 돌연변이.  |  Futreal, PA., et al. 1994. Science. 266: 120-2. PMID: 7939630
  8. 유방암 유전자는 놀라움을 선사합니다.  |  Nowak, R. 1994. Science. 265: 1796-9. PMID: 8091205
  9. 생체 내에서 BRCA1 유전자 산물과 상호 작용할 수 있는 RING 단백질의 확인.  |  Wu, LC., et al. 1996. Nat Genet. 14: 430-40. PMID: 8944023
  10. 개별 핵 영역에서 BARD1과 BRCA1 단백질의 세포 주기 의존적 공동화.  |  Jin, Y., et al. 1997. Proc Natl Acad Sci U S A. 94: 12075-80. PMID: 9342365
  11. 원발성 유방암, 난소암 및 자궁암에서 BRCA1 관련 RING 도메인(BARD1) 유전자의 돌연변이.  |  Thai, TH., et al. 1998. Hum Mol Genet. 7: 195-202. PMID: 9425226
  12. BAP1: 새로운 유비퀴틴 가수분해 효소로, BRCA1 링 핑거에 결합하여 BRCA1 매개 세포 성장 억제를 강화합니다.  |  Jensen, DE., et al. 1998. Oncogene. 16: 1097-112. PMID: 9528852

주문정보

제품명카탈로그 번호 단위가격수량관심품목

BAP1 항체 (C-4)

sc-28383
200 µg/ml
$316.00

BAP1 (C-4): m-IgG Fc BP-HRP 번들

sc-528441
200 µg Ab; 10 µg BP
$354.00

BAP1 (C-4): m-IgGκ BP-HRP 번들

sc-520819
200 µg Ab, 40 µg BP
$354.00

BAP1 (C-4): m-IgG1 BP-HRP 번들

sc-542933
200 µg Ab; 20 µg BP
$354.00

BAP1 항체 (C-4) AC

sc-28383 AC
500 µg/ml, 25% agarose
$416.00

BAP1 항체 (C-4) HRP

sc-28383 HRP
200 µg/ml
$316.00

BAP1 항체 (C-4) FITC

sc-28383 FITC
200 µg/ml
$330.00

BAP1 항체 (C-4) PE

sc-28383 PE
200 µg/ml
$343.00

BAP1 항체 (C-4) Alexa Fluor® 488

sc-28383 AF488
200 µg/ml
$357.00

BAP1 항체 (C-4) Alexa Fluor® 546

sc-28383 AF546
200 µg/ml
$357.00

BAP1 항체 (C-4) Alexa Fluor® 594

sc-28383 AF594
200 µg/ml
$357.00

BAP1 항체 (C-4) Alexa Fluor® 647

sc-28383 AF647
200 µg/ml
$357.00

BAP1 항체 (C-4) Alexa Fluor® 680

sc-28383 AF680
200 µg/ml
$357.00

BAP1 항체 (C-4) Alexa Fluor® 790

sc-28383 AF790
200 µg/ml
$357.00

BAP1- clone C-4 comes with predilute.

Asked by: Ronald Advincula
Thank you for your question. The antibody does not come pre-diluted .
Answered by: BlakeJ
Date published: 2024-04-02

Hei, I need SDS-Sheet for BAP1, C4, SC-28383

Asked by: SSHF KRS
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2024-04-01

Which BAP-1 antibody would be best for IHC-staining in uveal melanoma (human tissue)? Is there a protocol we could use?

Asked by: KHoesel
Thank you for your question. BAP1 Antibody (C-4): sc-28383 is the only antibody we currently offer that is recommended for both IHC applications and human tissues and cells. You can find our recommended protocol for IHC staining by following this link: https://www.scbt.com/scbt/de/resources/protocols/immunoperoxidase-staining Feel free to also contact our technical service team anytime for further assistance.
Answered by: Tech Support Europe
Date published: 2020-06-25

Do you know if this antibody will work for zebrafish?

Asked by: abc123
Thank you for your question. This antibody has not yet been tested in zebrafish and so that is not a recommended species at this time. We are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2020-01-02

What codons of the BAP1 protein is this antibody detecting?

Asked by: Kim Monkhorst
Thank you for your question. The epitope for this antibody maps within amino acids 430-729 of BAP 1 of human origin (accession#Q92560). The exact epitope has not been mapped.
Answered by: Tech Service
Date published: 2023-11-27

What is a good control tissue to use for BAP1  immunohistochemistry optimisation?

Asked by: GWAT1
Thank you for your question. Any tissue that expressed BAP1 would be a good control. We suggest duodenum or testis tissue as suitable controls.
Answered by: Tech Service
Date published: 2019-06-03

Hi, I'm having difficulty optimizing lot H0417. I are experiencing excessive background staining. (We ran our on Ultra Benchmark, CC1 and 64minutue, option 4 at 16 minute, Antibody incubation time is 1 hr, blocker). Please advise.

Asked by: IHC Tech
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-01-26

Dear, Is it possible to receive a free sample of the antibody BAP-1 (clone C4) refn° sc-28383

Asked by: doris peters
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-14
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Rated 2 out of 5 by from Inconsistent staining results of BAP1 AntibodyThis antibody produces high background signal on immunostaining. Although I tried to optimize the conditions, the background was still high. Although the efficiency of antibody varies among different batches. I tried 3 different batches; only one worked but produced high background, while the other two batches did not work at all in if, despite using the same conditions.
Date published: 2025-07-06
Rated 5 out of 5 by from works wellworks well for WB - used human cell lysates (HEK293T cells)
Date published: 2022-12-27
Rated 1 out of 5 by from 抗体染不上色!我买了这个BAP1 C4抗体,然而免疫组化做了好几次都染不上色,已经用了最 大浓度了,技术支持也很差
Date published: 2021-12-18
Rated 2 out of 5 by from 中皮腫症例を染色したものの…Leica BOND Maxで50倍希釈、PAb15分、pH6,pH9のそれぞれで20分賦活しました。結果は芳しくなく、あまりpotentialを感じませんでした。
Date published: 2021-09-28
Rated 5 out of 5 by from Works well for WesternUsed in 1:500 dilution to detect BAP1 in HEK293 whole cell extracts
Date published: 2019-02-01
Rated 1 out of 5 by from Inconstant staining resultsWith one batch (test sample), we obtained plausible staining results (Ventana; CC1/32min, 8' amplification, dilution 1:75/32min; nuclear loss in the tumor cells with mutation; nuclear expression in vascular endothelia and leukocytes as well as in tumors without mutation). With another batch (regularly ordered) identical staining procedures revealed strong background staining, which remained even at much higher dilutions of the antibody even in a mutated case.
Date published: 2018-02-27
Rated 4 out of 5 by from BAP1 trialI optimised this antibody for diagnosis of BAPoma on skin biopsy. I achieved good staining results using the trial sample and the lab will be adding this antibody to our repertoire in the near future.
Date published: 2017-11-24
Rated 5 out of 5 by from good antibodyI got this antibody as an sample to test it in prostate cancer cell line and found the antibody is good for WB 1:2000 dilution.
Date published: 2017-09-04
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BAP1 항체 (C-4) is rated 4.1 out of 5 by 17.
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