Ankyrin-1 Antibody (D-3): sc-374105

Ankyrin-1 Antibody (D-3) is a mouse monoclonal IgG1 antibody that detects Ankyrin-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Ankyrin-1 is a crucial membrane protein that plays a significant role in linking the cytoskeleton to the plasma membrane, particularly in erythrocytes, cardiac and skeletal muscle, and brain tissues. Anti-Ankyrin-1 antibody (D-3) recognizes a protein structure characterized by an N-terminal domain containing multiple ankyrin repeats, a highly conserved central spectrin binding domain, and C-terminal regulatory domains that exhibit considerable variability. This structural arrangement enables Ankyrin-1 to effectively anchor integral membrane proteins to the cytoskeleton, maintaining cellular integrity and facilitating communication between the cytoskeleton and plasma membrane. Ankyrin-1 exists in various isoforms, including a full-length protein and several shorter variants, which can influence functional properties. Defects in Ankyrin-1 have been implicated in hereditary spherocytosis, a common form of hemolytic anemia, highlighting Ankyrin-1′s importance in maintaining red blood cell stability and overall health. Ankyrin-1 monoclonal antibody (D-3) serves as an invaluable tool for researchers studying cytoskeletal dynamics and related pathologies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Ankyrin-1 Antibody (D-3) References:

1. Transient receptor potential ankyrin 1: emerging pharmacology and indications for cardiovascular biology.  |  Bodkin, JV. and Brain, SD. 2011. Acta Physiol (Oxf). 203: 87-98. PMID: 20950342
2. Transient Receptor Potential Ankyrin 1 (TRPA1) Channel and Neurogenic Inflammation in Pathogenesis of Asthma.  |  Yang, H. and Li, S. 2016. Med Sci Monit. 22: 2917-23. PMID: 27539812
3. Transient Receptor Potential Ankyrin 1 (TRPA1)-An Inflammation-Induced Factor in Human HaCaT Keratinocytes.  |  Luostarinen, S., et al. 2021. Int J Mol Sci. 22: PMID: 33805042
4. Activation of Transient Receptor Potential Ankyrin 1 and Vanilloid 1 Channels Promotes Odontogenic Differentiation of Human Dental Pulp Cells.  |  Lou, Y., et al. 2021. J Endod. 47: 1409-1416. PMID: 34126160
5. Functional Transient Receptor Potential Ankyrin 1 and Vanilloid 1 Ion Channels Are Overexpressed in Human Oral Squamous Cell Carcinoma.  |  Kiss, F., et al. 2022. Int J Mol Sci. 23: PMID: 35163843
6. Species-Specific Activation of Transient Receptor Potential Ankyrin 1 by Phthalic Acid Monoesters.  |  Mori, Y., et al. 2022. Biol Pharm Bull. 45: 1839-1846. PMID: 36223942
7. Transient receptor potential Ankyrin-1 (TRPA1) agonists suppress myelination and induce demyelination in organotypic cortical slices.  |  Giacco, V., et al. 2023. Glia. 71: 1402-1413. PMID: 36762504
8. Transient Receptor Potential Ankyrin 1 (TRPA1) Channel Mediates Acrolein Cytotoxicity in Human Lung Cancer Cells.  |  Sakamoto, A., et al. 2023. Int J Mol Sci. 24: PMID: 37511605
9. Human Transient Receptor Potential Ankyrin 1 Channel: Structure, Function, and Physiology.  |  Vlachova, V., et al. 2024. Subcell Biochem. 104: 207-244. PMID: 38963489
10. Ankyrin-1 mutations are a major cause of dominant and recessive hereditary spherocytosis.  |  Eber, SW., et al. 1996. Nat Genet. 13: 214-8. PMID: 8640229

The preferred Ankyrin-1 antibody is Ankyrin-1 Antibody (8C3): sc-12733.