α_1A-AR Antibody (4D8): sc-100291

α1A-AR Antibody (4D8) is a mouse monoclonal IgG1 kappa light chain antibody that detects α1A-AR protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-α1A-AR antibody (4D8) is available as a non-conjugated format. α1A-AR plays a crucial role in the sympathetic nervous system by mediating catecholamine effects such as epinephrine and norepinephrine, which regulate cardiovascular functions. α1A-AR couples with Gq/11 proteins, leading to vasoconstriction and increased blood pressure, influencing vascular tone and cardiac output. α1A-AR expression spans across heart, brain, liver, and prostate tissues, where alternative splicing of ADRA1A gene results in multiple isoforms with unique C-termini showing distinct activation patterns. α1A-AR transcripts in heart, liver, and prostate, ranging from 2.0 to 6.0 kb, highlight significant roles in physiological processes and potential implications in pathophysiological conditions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

α_1A-AR Antibody (4D8) References:

1. alpha 1-Adrenergic receptor subtypes differentially control the cell cycle of transfected CHO cells through a cAMP-dependent mechanism involving p27Kip1.  |  Shibata, K., et al. 2003. J Biol Chem. 278: 672-8. PMID: 12409310
2. Differential regulation of the cell cycle by alpha1-adrenergic receptor subtypes.  |  Gonzalez-Cabrera, PJ., et al. 2004. Endocrinology. 145: 5157-67. PMID: 15297446
3. Beta-arrestin-dependent spontaneous alpha1a-adrenoceptor endocytosis causes intracellular transportation of alpha-blockers via recycling compartments.  |  Pediani, JD., et al. 2005. Mol Pharmacol. 67: 992-1004. PMID: 15626751
4. Mammalian tolloid alters subcellular localization, internalization, and signaling of alpha(1a)-adrenergic receptors.  |  Xu, Q., et al. 2006. Mol Pharmacol. 70: 532-41. PMID: 16690783
5. Real-time detection of alpha1A-AR movement stimulated by phenylephrine in single living cells.  |  Xu, N., et al. 2007. Acta Pharmacol Sin. 28: 796-802. PMID: 17506938
6. Evidence of alpha1-adrenoceptor functional changes in omental arteries of patients with end-stage renal disease.  |  Cruz-Domínguez, MP., et al. 2008. Auton Autacoid Pharmacol. 28: 19-27. PMID: 18257748
7. GFAP and alpha1a-AR staining and nuclear morphometry of oligodendrogliomas by confocal microscopy and image analysis: useful parameters for predicting survival in oligodendrogliomas.  |  Moro-Rodríguez, E., et al. 2008. Diagn Pathol. 3 Suppl 1: S26. PMID: 18673515
8. Alpha 1-adrenoceptor pharmacome: alpha 1L-adrenoceptor and alpha 1A-adrenoceptor in the lower urinary tract.  |  Nishimune, A., et al. 2010. Int J Urol. 17: 31-7. PMID: 19694838
9. Carotid dysfunction in senescent female mice is mediated by increased α1A-adrenoceptor activity and COX-derived vasoconstrictor prostanoids.  |  Costa, TJ., et al. 2023. Am J Physiol Heart Circ Physiol. 324: H417-H429. PMID: 36705993
10. Cloning, functional expression and tissue distribution of human cDNA for the alpha 1C-adrenergic receptor.  |  Hirasawa, A., et al. 1993. Biochem Biophys Res Commun. 195: 902-9. PMID: 8396931
11. Molecular cloning, genomic characterization and expression of novel human alpha1A-adrenoceptor isoforms.  |  Chang, DJ., et al. 1998. FEBS Lett. 422: 279-83. PMID: 9490024
12. Examination of alpha 1 adrenoceptor subtypes in the human ureter.  |  Itoh, Y., et al. 2007. Int J Urol. 14: 749-53. PMID: 17681068

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-α_1A-AR Antibody (4D8) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-α_1A-AR Antibody (4D8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

α_1A-AR Antibody (4D8) Specifications

Alternate Product Name: ADRA1A

Product Clone Name: 4D8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-28

Epitope Target: α_1A-AR

Epitope Species: human

Product Reactivity/Detection: α_1A-AR

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for α_1A-AR Antibody (4D8): N/A