α/β-centractin Antibody (A-7): sc-376010
- α/β-centractin Antibody (A-7) is a mouse monoclonal IgG1 κ α/β-centractin antibody provided at 200 µg/ml
- raised against amino acids 77-376 mapping at the C-terminus of α-centractin of human origin
- alpha / beta-centractin Antibody (A-7) is recommended for detection of α-centractin and β-centractin of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-alpha / beta-centractin Antibody (A-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for α/β-centractin Antibody (A-7) for WB applications. This reagent is now offered in a bundle with α/β-centractin Antibody (A-7) (see ordering information below).
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α/β-centractin Antibody (A-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects α/β-centractin of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). α/β-centractin (A-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Centractin, a crucial component of the dynactin complex, plays a significant role in cellular processes such as organelle transport, mitosis, and maintaining cellular architecture. The dynactin complex, which consists of 10-11 distinct subunits, is essential for the proper functioning of dynein, a motor protein responsible for retrograde transport along microtubules. Centractin exists in two isoforms, α and β, with the α isoform, also known as actin-related protein 1 homolog A (Arp1), being the most prevalent. The β isoform, or actin-related protein 1 homolog B, shares a remarkable 90% sequence identity with the α isoform, and these two isoforms are expressed in a ratio of approximately 15:1. The structural integrity of the dynactin complex is vital, as this complex facilitates the arrangement and interaction of other proteins within the complex, thereby influencing various cellular functions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
α/β-centractin Antibody (A-7) References:
- Self-regulated polymerization of the actin-related protein Arp1. | Bingham, JB. and Schroer, TA. 1999. Curr Biol. 9: 223-6. PMID: 10074429
- Assignment of beta-centractin (CTRN2) to human chromosome 2 bands q11.1-->q11.2 with somatic cell hybrids and in situ hybridization. | Elsea, SH., et al. 1999. Cytogenet Cell Genet. 84: 48-9. PMID: 10343100
- Dynactin is necessary for synapse stabilization. | Eaton, BA., et al. 2002. Neuron. 34: 729-41. PMID: 12062020
- Three-dimensional reconstruction of the dynactin complex by single-particle image analysis. | Hodgkinson, JL., et al. 2005. Proc Natl Acad Sci U S A. 102: 3667-72. PMID: 15738427
- Changes in cytoskeletal gene expression linked to MPTP-treatment in Mice. | Cuadrado-Tejedor, M., et al. 2005. Neurobiol Dis. 20: 666-72. PMID: 16005240
- Molecular dissection of ARP1 regions required for nuclear migration and cell wall integrity checkpoint functions in Saccharomyces cerevisiae. | Igarashi, R., et al. 2005. Cell Struct Funct. 30: 57-67. PMID: 16415535
- Activation of endosomal dynein motors by stepwise assembly of Rab7-RILP-p150Glued, ORP1L, and the receptor betalll spectrin. | Johansson, M., et al. 2007. J Cell Biol. 176: 459-71. PMID: 17283181
- Dynactin is required for coordinated bidirectional motility, but not for dynein membrane attachment. | Haghnia, M., et al. 2007. Mol Biol Cell. 18: 2081-9. PMID: 17360970
- Beta-centractin: characterization and distribution of a new member of the centractin family of actin-related proteins. | Clark, SW., et al. 1994. Mol Biol Cell. 5: 1301-10. PMID: 7696711
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
α/β-centractin Antibody (A-7) | sc-376010 | 200 µg/ml | $322.00 | |||
α/β-centractin Antibody (A-7): m-IgG Fc BP-HRP Bundle | sc-552032 | 200 µg Ab; 10 µg BP | $361.00 | |||
α/β-centractin Antibody (A-7) AC | sc-376010 AC | 500 µg/ml, 25% agarose | $424.00 | |||
α/β-centractin Antibody (A-7) HRP | sc-376010 HRP | 200 µg/ml | $322.00 | |||
α/β-centractin Antibody (A-7) FITC | sc-376010 FITC | 200 µg/ml | $336.00 | |||
α/β-centractin Antibody (A-7) PE | sc-376010 PE | 200 µg/ml | $349.00 | |||
α/β-centractin Antibody (A-7) Alexa Fluor® 488 | sc-376010 AF488 | 200 µg/ml | $364.00 | |||
α/β-centractin Antibody (A-7) Alexa Fluor® 546 | sc-376010 AF546 | 200 µg/ml | $364.00 | |||
α/β-centractin Antibody (A-7) Alexa Fluor® 594 | sc-376010 AF594 | 200 µg/ml | $364.00 | |||
α/β-centractin Antibody (A-7) Alexa Fluor® 647 | sc-376010 AF647 | 200 µg/ml | $364.00 | |||
α/β-centractin Antibody (A-7) Alexa Fluor® 680 | sc-376010 AF680 | 200 µg/ml | $364.00 | |||
α/β-centractin Antibody (A-7) Alexa Fluor® 790 | sc-376010 AF790 | 200 µg/ml | $364.00 |