Akt3 항체 (EE-M14) WB, IP와 ELISA으로 mouse, rat와 human origin의 Akt3을 검출할것을 권장합니다.

Akt3 항체 (EE-M14): sc-134254

Akt3 항체 (EE-M14) is rated 4.6 out of 5 by 8.
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데이터 시트

    • Akt3 항체(EE-M14) 는 마우스 monoclonal IgG2aκ, 4간행물에 인용, 이며 100 µg/ml으로 제공합니다.
    • raised against recombinant Akt3 protein of human origin
    • WB, IP와 ELISA으로 mouse, rat와 human origin의 Akt3을 검출할것을 권장합니다.
    • Akt3 (EE-M14): sc-134254무료10 µg 샘플을 신청하려면 기술지원팀 (또는 로컬 디스트리뷰터)에 연락주세요.

For Western Blot, is it recommended to use denatured or non-denatured conditions with Akt3 (EE-M14): sc-134254 antibody?

Asked by: Randy McDonald
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 4 out of 5 by from Worked for WB Gave a good band after longer exposure time. Used 1:200 for mouse tissue lysates
Date published: 2018-03-23
Rated 4 out of 5 by from Good antibody Worked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 4 out of 5 by from a little weak this antibody is specific , but the band is a little weak.
Date published: 2017-07-07
Rated 5 out of 5 by from Works well for Western with HSC-3 cells WB: 1:200. Single strong band with HSC-3 and cal-27 cells.
Date published: 2017-01-10
Rated 5 out of 5 by from Single strong band in mouse NIH/3T3 samples Single strong band in mouse NIH/3T3 samples. -SCBT QC
Date published: 2015-03-24
Rated 5 out of 5 by from Works well for Western in MCF7 cells Works well for Western in MCF7 cells. -SCBT QC
Date published: 2015-02-26
Rated 5 out of 5 by from Great WB data in HeLa cells Great WB data in HeLa cells. -SCBT QC
Date published: 2015-02-06
Rated 5 out of 5 by from Clean WB Clean WB, single band in K-562. -SCBT QC
Date published: 2015-01-29
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