Akp-3 CRISPR/Cas9 KO Plasmid (m): sc-419069
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Akp-3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Akp-3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Akp-3 CRISPR/Cas9 KO Plasmid (m) | sc-419069 | 20 µg | $397.00 |
Overview
Akp3 encodes the mouse alkaline phosphatase 3 (Akp-3), a glycosylphosphatidylinositol-anchored ectoenzyme that hydrolyzes phosphate monoesters at the cell surface and in extracellular compartments. By dephosphorylating nucleotides and other phospho-metabolites, Akp-3 can influence local phosphate availability, purinergic signaling tone, and broader metabolic homeostasis. Alkaline phosphatase activity is commonly linked to epithelial differentiation and tissue-specific barrier and absorptive functions, supporting studies of organ physiology and cellular maturation programs. Altered alkaline phosphatase expression or activity is frequently used as a readout in models of inflammation, metabolic dysregulation, and differentiation-associated pathologies, making Akp3 a relevant target for mechanistic investigation.
Akp-3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Akp3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Akp3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Akp3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Akp-3 protein expression.
This CRISPR knockout system enables efficient generation of Akp3-deficient cell models for investigation of Akp-3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Akp3 exon(s) critical for Akp-3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Akp3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Akp-3 CRISPR/Cas9 KO Plasmid (m) and Akp-3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Akp3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Akp-3 HDR Plasmid (m) and Akp-3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Akp3 homology arms to support homology-directed repair at defined Akp3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.