ACOT1/2 Antibody (E-1): sc-373919

ACOT1/2 Antibody (E-1) is a mouse monoclonal IgG2b antibody that detects acyl-CoA thioesterase 1/2 (ACOT1/2) in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). ACOT1 and ACOT2 play crucial roles in lipid metabolism by catalyzing the hydrolysis of acyl-CoA to produce coenzyme A and free fatty acids, thereby regulating intracellular levels of fatty acids and acyl-CoAs, which is vital for maintaining cellular energy homeostasis and signaling pathways. Notably, ACOT1 is predominantly found in the cytoplasm as a monomer, while ACOT2 is primarily localized in the mitochondria, highlighting their distinct functional roles within the cell. The differential localization of these enzymes is significant as it suggests that they may participate in separate metabolic processes, with ACOT2 potentially influencing mitochondrial fatty acid metabolism and energy production. Additionally, ACOT2 is known to exist in multiple alternatively spliced isoforms, which may further diversify its functional capabilities and regulatory mechanisms. ACOT1/2 (E-1) antibody is an essential tool for researchers studying lipid metabolism and its implications in various physiological and pathological conditions.

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ACOT1/2 Antibody (E-1) References:

1. The acyl-CoA thioesterase I is regulated by PPARalpha and HNF4alpha via a distal response element in the promoter.  |  Dongol, B., et al. 2007. J Lipid Res. 48: 1781-91. PMID: 17485727
2. Protective effects of Acyl-coA thioesterase 1 on diabetic heart via PPARα/PGC1α signaling.  |  Yang, S., et al. 2012. PLoS One. 7: e50376. PMID: 23226270
3. Cardiomyocyte specific expression of Acyl-coA thioesterase 1 attenuates sepsis induced cardiac dysfunction and mortality.  |  Xia, C., et al. 2015. Biochem Biophys Res Commun. 468: 533-40. PMID: 26518651
4. Acyl-CoA Thioesterase 1 (ACOT1) Regulates PPARα to Couple Fatty Acid Flux With Oxidative Capacity During Fasting.  |  Franklin, MP., et al. 2017. Diabetes. 66: 2112-2123. PMID: 28607105
5. Metallo-β-lactamase domain-containing protein 2 is S-palmitoylated and exhibits acyl-CoA hydrolase activity.  |  Malgapo, MIP., et al. 2021. J Biol Chem. 296: 100106. PMID: 33219126
6. Difference of copy number variation in blood of patients with lung cancer.  |  Heo, Y., et al. 2021. Int J Biol Markers. 36: 3-9. PMID: 33307925
7. Weighted Gene Co-Expression Network Analysis Identifies ANGPTL4 as a Key Regulator in Diabetic Cardiomyopathy via FAK/SIRT3/ROS Pathway in Cardiomyocyte.  |  Dai, L., et al. 2021. Front Endocrinol (Lausanne). 12: 705154. PMID: 34616362
8. Intestinal farnesoid X receptor signaling controls hepatic fatty acid oxidation.  |  Lu, D., et al. 2022. Biochim Biophys Acta Mol Cell Biol Lipids. 1867: 159089. PMID: 34856412
9. Icariin and Competing Endogenous RNA Network: A Potential Protective Strategy Against Contrast-Induced Acute Kidney Injury.  |  Lin, Y., et al. 2022. Drug Des Devel Ther. 16: 2343-2363. PMID: 35910780
10. Regulation of cardiac ferroptosis in diabetic human heart failure: uncovering molecular pathways and key targets.  |  Gawargi, FI. and Mishra, PK. 2024. Cell Death Discov. 10: 268. PMID: 38824159

The preferred ACOT1/2 antibody is ACOT1/2 Antibody (F-2): sc-373917.