TRAP-β Antibody (AT31G6): sc-517428

TRAP-β Antibody (AT31G6) is a mouse monoclonal IgG1 kappa light chain antibody that detects TRAP-beta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Anti-TRAP-beta antibody (AT31G6) is available as the non-conjugated format. The TRAP proteins, which include TRAP-α, TRAP-β, TRAP-γ, and TRAP-δ, are essential transmembrane proteins that form a heterotetramer complex known as the signal sequence receptor (SSR) or TRAP complex, localized primarily in the endoplasmic reticulum (ER). This localization is crucial as TRAP complex plays a significant role in the retention of ER resident proteins, ensuring proper protein folding and function. Additionally, TRAP complex interacts with the Sec61 translocon, the primary mediator of protein translocation across the ER membrane, thereby facilitating the import of nascent polypeptides into the ER. Furthermore, TRAP complex is implicated in ER-associated degradation (ERAD), a vital process for maintaining cellular homeostasis, particularly during ER stress, when TRAP complex subunits are induced by the XBP-1/IRE1α pathway. The structural composition of TRAP proteins, with TRAP-α, TRAP-β, and TRAP-δ being single-pass membrane proteins and TRAP-γ containing four transmembrane domains, underscores their functional diversity and importance in cellular processes.

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TRAP-β Antibody (AT31G6) References:

1. Oligomeric complexes involved in translocation of proteins across the membrane of the endoplasmic reticulum.  |  Wang, L. and Dobberstein, B. 1999. FEBS Lett. 457: 316-22. PMID: 10471800
2. The Translocon-Associated Protein beta (TRAPbeta) in zebrafish embryogenesis. I. Enhanced expression of transcripts in notochord and hatching gland precursors.  |  Mangos, S., et al. 2000. Mol Cell Biochem. 215: 93-101. PMID: 11204460
3. Substrate-specific function of the translocon-associated protein complex during translocation across the ER membrane.  |  Fons, RD., et al. 2003. J Cell Biol. 160: 529-39. PMID: 12578908
4. Cloning and molecular characterization of a human ortholog of Monodelphis TRAPD in ultraviolet B-induced melanoma.  |  Wang, Z. and VandeBerg, JL. 2004. Melanoma Res. 14: 107-14. PMID: 15057039
5. Architecture of the ribosome-channel complex derived from native membranes.  |  Ménétret, JF., et al. 2005. J Mol Biol. 348: 445-57. PMID: 15811380
6. Mutation in the Trapalpha/Ssr1 gene, encoding translocon-associated protein alpha, results in outflow tract morphogenetic defects.  |  Mesbah, K., et al. 2006. Mol Cell Biol. 26: 7760-71. PMID: 17015483
7. Simultaneous induction of the four subunits of the TRAP complex by ER stress accelerates ER degradation.  |  Nagasawa, K., et al. 2007. EMBO Rep. 8: 483-9. PMID: 17380188
8. A tetrameric complex of membrane proteins in the endoplasmic reticulum.  |  Hartmann, E., et al. 1993. Eur J Biochem. 214: 375-81. PMID: 7916687
9. Genomic organization of two novel genes on human Xq28: compact head to head arrangement of IDH gamma and TRAP delta is conserved in rat and mouse.  |  Brenner, V., et al. 1997. Genomics. 44: 8-14. PMID: 9286695

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TRAP-β Antibody (AT31G6) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-TRAP-β Antibody (AT31G6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TRAP-β Antibody (AT31G6) Specifications

Alternate Product Name: TRAP-β

Product Clone Name: AT31G6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 18-149

Epitope Target: TRAP-β

Epitope Species: human

Product Reactivity/Detection: TRAP-β

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide, 1% glycerol and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for TRAP-β Antibody (AT31G6): N/A