Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Tom20 CRISPR/Cas9 KO Plasmid (h): sc-400041
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Tom20 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Tom20 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Tom20 Antibody (F-10): sc-17764
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Tom20 CRISPR/Cas9 KO Plasmid (h) | sc-400041 | 20 µg | $397.00 |
Overview
TOMM20 encodes Tom20, a core receptor of the translocase of the outer mitochondrial membrane (TOM) complex that recognizes N-terminal mitochondrial targeting sequences and initiates import of nuclear-encoded proteins into mitochondria. By governing mitochondrial protein biogenesis, Tom20 influences oxidative phosphorylation, mitochondrial dynamics, mitophagy, and stress signaling pathways that shape cellular metabolism and survival. Altered TOMM20 expression or mitochondrial import efficiency has been linked to mitochondrial dysfunction observed across neurodegeneration, cardiometabolic disorders, and cancer-associated metabolic remodeling. Tom20 is therefore widely used as a functional node for studying mitochondrial homeostasis, proteostasis, and organelle-to-nucleus communication.
Tom20 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TOMM20 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TOMM20 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TOMM20 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Tom20 protein expression.
This CRISPR knockout system enables efficient generation of TOMM20-deficient cell models for investigation of Tom20 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TOMM20 exon(s) critical for Tom20 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TOMM20 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Tom20 CRISPR/Cas9 KO Plasmid (h) and Tom20 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TOMM20 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Tom20 HDR Plasmid (h) and Tom20 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TOMM20 homology arms to support homology-directed repair at defined TOMM20 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.