Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TGF beta 1 Double Nickase Plasmid (h): sc-400067-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TGF beta 1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TGF beta 1 Double Nickase Plasmid (h) and TGF beta 1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TGFB1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TGF beta 1 Antibody (3C11): sc-130348
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TGF beta 1 Double Nickase Plasmid (h) | sc-400067-NIC | 20 µg | $410.00 | |||
TGF beta 1 Double Nickase Plasmid (h2) | sc-400067-NIC-2 | 20 µg | $410.00 |
TGFB1 encodes transforming growth factor beta 1 (TGF beta 1), a secreted cytokine that signals through TGFBR1/2 to activate SMAD2/3-dependent transcription and intersect with MAPK, PI3K/AKT, and Rho GTPase pathways. It is a central regulator of extracellular matrix remodeling, epithelial–mesenchymal transition, immune suppression, and wound-healing programs, influencing cell-cycle arrest and differentiation in a context-dependent manner. Dysregulated TGF beta 1 signaling is implicated in fibrotic disorders, tumor microenvironment remodeling, and aberrant inflammation, making TGFB1 a common node for studying crosstalk between stromal and immune compartments.
TGF beta 1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TGFB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TGFB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TGFB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TGFB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.