TFIIA-α/β Antibody (708C4a): sc-81398

TFIIA-α/β Antibody (708C4a) is a mouse monoclonal IgG1 antibody that detects TFIIA-α/β of mouse, rat, and human origin by western blotting (WB) and immunoprecipitation (IP). TFIIA-α/β (708C4a) antibody is available as a non-conjugated TFIIA-α/β monoclonal isotype antibody. TFIIA-α/β plays a crucial role in initiating transcription from protein-coding genes in eukaryotes, a process essential for gene expression and cellular function. TFIIA-α/β consists of three subunits: TFIIA-α, TFIIA-β, and TFIIA-γ, which work together to stabilize interaction between TATA-binding protein (TBP) and DNA at the TATA box. This stabilization facilitates preinitiation complex (PIC) formation, necessary for recruiting RNA polymerase II and other transcription factors. By binding directly to TBP and TATA box, TFIIA-α/β enhances transcriptional output of genes, making TFIIA-α/β a key player in gene expression regulation. TFIIA-α/β′s ability to modulate TATA binding element recognition by TBP underscores TFIIA-α/β′s importance in transcriptional machinery, influencing various cellular processes and responses.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TFIIA-α/β Antibody (708C4a) References:

1. TFIIAalpha/beta-like factor is encoded by a germ cell-specific gene whose expression is up-regulated with other general transcription factors during spermatogenesis in the mouse.  |  Han, SY., et al. 2001. Biol Reprod. 64: 507-17. PMID: 11159353
2. TFIIA plays a role in the response to oxidative stress.  |  Kraemer, SM., et al. 2006. Eukaryot Cell. 5: 1081-90. PMID: 16835452
3. Factors involved in specific transcription by mammalian RNA polymerase II. Identification and characterization of factor IIH.  |  Flores, O., et al. 1992. J Biol Chem. 267: 2786-93. PMID: 1733973
4. TFIIA changes the conformation of the DNA in TBP/TATA complexes and increases their kinetic stability.  |  Hieb, AR., et al. 2007. J Mol Biol. 372: 619-32. PMID: 17681538
5. Nonradioactive, ultrasensitive site-specific protein-protein photocrosslinking: interactions of alpha-helix 2 of TATA-binding protein with general transcription factor TFIIA and transcriptional repressor NC2.  |  Kim, Y., et al. 2008. Nucleic Acids Res. 36: 6143-54. PMID: 18824481
6. Factors involved in specific transcription by mammalian RNA polymerase II: role of transcription factors IIA, IID, and IIB during formation of a transcription-competent complex.  |  Maldonado, E., et al. 1990. Mol Cell Biol. 10: 6335-47. PMID: 2247058
7. An RNA polymerase II transcription factor has an associated DNA-dependent ATPase (dATPase) activity strongly stimulated by the TATA region of promoters.  |  Conaway, RC. and Conaway, JW. 1989. Proc Natl Acad Sci U S A. 86: 7356-60. PMID: 2552440
8. Taspase1 processing alters TFIIA cofactor properties in the regulation of TFIID.  |  Malecová, B., et al. 2015. Transcription. 6: 21-32. PMID: 25996597
9. Five intermediate complexes in transcription initiation by RNA polymerase II.  |  Buratowski, S., et al. 1989. Cell. 56: 549-61. PMID: 2917366
10. Factors involved in specific transcription by mammalian RNA polymerase II: purification, genetic specificity, and TATA box-promoter interactions of TFIID.  |  Nakajima, N., et al. 1988. Mol Cell Biol. 8: 4028-40. PMID: 3185540
11. Human general transcription factor TFIIA: characterization of a cDNA encoding the small subunit and requirement for basal and activated transcription.  |  DeJong, J., et al. 1995. Proc Natl Acad Sci U S A. 92: 3313-7. PMID: 7724559
12. Molecular cloning of the small (gamma) subunit of human TFIIA reveals functions critical for activated transcription.  |  Ozer, J., et al. 1994. Genes Dev. 8: 2324-35. PMID: 7958899

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TFIIA-α/β Antibody (708C4a) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-TFIIA-α/β Antibody (708C4a) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TFIIA-α/β Antibody (708C4a) Specifications

Alternate Product Name: TFIIA-α/β

Product Clone Name: 708C4a

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: TFIIA-α/β

Epitope Species: human

Product Reactivity/Detection: TFIIA-α (35 kDa) of mouse, rat and human origin, and TFIIA-β (19 kDa) of human origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS containing 0.1% sodium azide and 0.2% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for TFIIA-α/β Antibody (708C4a): N/A