TBX4 Double Nickase Plasmid (h): sc-405242-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TBX4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TBX4 Double Nickase Plasmid (h) and TBX4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TBX4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TBX4 Antibody (G-4): sc-515196
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TBX4 Double Nickase Plasmid (h) | sc-405242-NIC | 20 µg | $410.00 | |||
TBX4 Double Nickase Plasmid (h2) | sc-405242-NIC-2 | 20 µg | $410.00 |
TBX4 encodes a T-box family transcription factor that binds DNA through a conserved T-box domain to regulate gene expression programs controlling embryonic mesenchymal differentiation, limb patterning, and organogenesis. In human development, TBX4 activity integrates with WNT, BMP, and FGF signaling networks to coordinate progenitor specification and tissue morphogenesis. Dysregulated TBX4 function is implicated in congenital developmental phenotypes, including limb malformations and pulmonary vascular disease, making it a relevant target for studying transcriptional control of developmental pathways. As a nuclear regulator, TBX4 provides a tractable node for investigating enhancer–promoter logic, lineage decisions, and downstream transcriptional circuits in relevant cell models.
TBX4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TBX4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TBX4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TBX4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TBX4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.