SRCR Antibody (5F10): sc-73522

SRCR Antibody (5F10) is a mouse monoclonal IgM antibody that detects the SRCR protein of Geodia cydonium origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-SRCR antibody (5F10) is available as the non-conjugated format, specifically designed for research involving the Aggregation Factor, a crucial protein extensively studied in various sponge species, including Geodia cydonium. The Aggregation Factor plays a vital role in cellular adhesion and communication, which is essential for the structural integrity and functionality of sponge tissues. This protein is composed of multiple glycoproteins that form a complex structure around a 90S sunburst-like core, facilitating the bridging of two cells through the ligation of a cell-surface-bound Aggregation Receptor. The Aggregation Receptor, which contains scavenger receptor cysteine-rich (SRCR) domains, is integral to the interaction with cell surface integrins, thereby influencing the adhesive properties of cells. Understanding the function of the Aggregation Factor and its interactions is critical, as it provides insights into the evolutionary biology of multicellular organisms and the mechanisms underlying cell adhesion and tissue organization.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SRCR Antibody (5F10) References:

1. Cell adhesion and histocompatibility in sponges.  |  Fernàndez-Busquets, X. and Burger, MM. 1999. Microsc Res Tech. 44: 204-18. PMID: 10098923
2. Cloning and expression of the putative aggregation factor from the marine sponge Geodia cydonium.  |  Schütze, J., et al. 2001. J Cell Sci. 114: 3189-98. PMID: 11590245
3. The Scavenger Receptor Cysteine-Rich (SRCR) domain: an ancient and highly conserved protein module of the innate immune system.  |  Sarrias, MR., et al. 2004. Crit Rev Immunol. 24: 1-37. PMID: 14995912
4. Adhesion forces in the self-recognition of oligosaccharide epitopes of the proteoglycan aggregation factor of the marine sponge Microciona prolifera.  |  Carvalho de Souza, A., et al. 2009. Glycoconj J. 26: 457-65. PMID: 18843533
5. Sponge aggregation factor: identification of the specific collagen-binding site by means of a monoclonal antibody.  |  Gramzow, M., et al. 1988. J Histochem Cytochem. 36: 205-12. PMID: 3335775
6. A galectin links the aggregation factor to cells in the sponge (Geodia cydonium) system.  |  Wagner-Hülsmann, C., et al. 1996. Glycobiology. 6: 785-93. PMID: 9023539
7. On the origin of Metazoan adhesion receptors: cloning of integrin alpha subunit from the sponge Geodia cydonium.  |  Pancer, Z., et al. 1997. Mol Biol Evol. 14: 391-8. PMID: 9100369
8. A novel member of an ancient superfamily: sponge (Geodia cydonium, Porifera) putative protein that features scavenger receptor cysteine-rich repeats.  |  Pancer, Z., et al. 1997. Gene. 193: 211-8. PMID: 9256079
9. The putative sponge aggregation receptor. Isolation and characterization of a molecule composed of scavenger receptor cysteine-rich domains and short consensus repeats.  |  Blumbach, B., et al. 1998. J Cell Sci. 111 (Pt 17): 2635-44. PMID: 9701562