Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SRA Double Nickase Plasmid (h): sc-404308-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SRA Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SRA Double Nickase Plasmid (h) and SRA Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SRA1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SRA Antibody (E-5): sc-393240
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SRA Double Nickase Plasmid (h) | sc-404308-NIC | 20 µg | $410.00 | |||
SRA Double Nickase Plasmid (h2) | sc-404308-NIC-2 | 20 µg | $410.00 |
SRA1 encodes steroid receptor RNA activator 1 (SRA), a multifunctional RNA and protein co-regulator that modulates transcriptional programs controlled by nuclear receptors and other transcription factors. SRA participates in chromatin remodeling and assembly of transcriptional complexes that influence hormone-responsive gene expression, coupling steroid signaling with broader epigenetic and RNA-mediated regulatory processes. Through these activities, SRA1 is linked to pathways governing cell differentiation, metabolic regulation, and stress-responsive transcriptional outputs. Dysregulated SRA1/SRA expression has been reported across hormone-related and proliferative disease contexts, supporting its use as a mechanistic target for dissecting transcriptional network perturbations.
SRA Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SRA1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SRA1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SRA1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SRA1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.