SPF30 Antibody (SQ-16): sc-100338

SPF30 Antibody (SQ-16) is a mouse monoclonal IgG1 antibody that detects SPF30 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). SPF30, also known as SMNDC1 or SMN-related protein, is a crucial splicing factor that plays a vital role in the assembly of the spliceosome, a complex responsible for the removal of introns from pre-mRNA. SPF30 is predominantly expressed in skeletal muscle, pancreas, and heart tissues, where SPF30 localizes to Cajal bodies and nuclear speckles, sites essential for RNA processing. The structure of SPF30 includes a Tudor domain that shares significant similarity with the Survival Motor Neuron (SMN) protein, highlighting SPF30′s importance in the splicing machinery. The interaction of SPF30 with five U small nuclear ribonucleoproteins (snRNPs) is critical for the recruitment of the U4/U5/U6 tri-snRNP to the prespliceosome, facilitating efficient spliceosome assembly. Loss of SPF30 function can lead to a halt in spliceosome assembly at the prespliceosome stage, which can have detrimental effects on gene expression and cellular function. Overexpression of SPF30 has been linked to apoptosis, underscoring SPF30′s dual role in cellular processes. With detection capabilities across multiple species and applications, anti-SPF30 antibody (SQ-16) serves as an invaluable tool for researchers studying splicing mechanisms and related pathologies.

The preferred SPF30 antibody is SPF30 Antibody (G-8): sc-376887.