RIPA Lysis Buffer System (w/out PMSF) A high quality, ready to use mammalian cell and tissue lysis buffer with protease inhibitors included.

RIPA Lysis Buffer System (w/out PMSF)

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RIPA Lysis Buffer System (w/out PMSF) is rated 5.0 out of 5 by 1.
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別名: RIPA Lysis Buffer System (w/out PMSF) also known as PMSF Protease Inhibitor
アプリケーション: RIPA Lysis Buffer System (w/out PMSF) is a high quality, ready to use mammalian cell and tissue lysis buffer with protease inhibitors included
試験・研究用以外には使用しないでください。 臨床及び体外診断には使用できません。
* Refer to Certificate of Analysis for lot specific data (including water content).
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RIPA (Radioimmunoprecipitation) Lysis Buffer (w/out PMSF) is used to lyse cells and tissue, for radio immunoprecipitation assay (RIPA). RIPA lysis buffer has stronger denaturing capabilities than NP-40 (sc-281108) or Triton X-100 (sc-29112) and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts.

RIPA Lysis Buffer System (w/out PMSF) is a high quality product that includes protease inhibitors, making it ready for use in mammalian cell and tissue lysis.

sc-364162, 50 mL - Components supplied in three vials:
VIAL 1: 50 mL 1X lysis buffer
VIAL 2: 500 μL protease inhibitor cocktail in DMSO
VIAL 3: 500 μL (100mM) sodium orthovanadate in water

sc-364162A, 500 mL - Components supplied in three vials:
VIAL 1: 500 mL 1X lysis buffer
VIAL 2: 5 ml protease inhibitor cocktail in DMSO
VIAL 3: 5 ml (100mM) sodium orthovanadate in water


参考文献

1. Poirier, F. et al. 1982. Int. J. Cancer. 29(1): 69-76. PMID: 6277805 2. DeSeau, V. et al. 1987. J. Cell. Biochem. 35(2): 113-128. PMID: 2448318 3. Bolen, J.B. et al. 1987. Oncogene Res. 1(2): 149-168. PMID: 2453014 4. Ngoka, L.C. 2008. Proteome Sci. 6

用法 :
• Immediately prior to lysing cells, combine 10 μl sodium orthovanadate solution and 10-20 μl protease inhibitor cocktail solution per ml of 1X RIPA Lysis buffer to prepare complete RIPA.

• Use 3 ml complete RIPA per gram of tissue

- OR 1 ml complete RIPA per 2.0x107 cells in suspension

- OR 0.6 ml complete RIPA per subconfluent monolayer on a 100 mm plate.
Physical State :
Liquid
保存方法 :
Store at -20° C
試験・研究用以外には使用しないでください。 臨床及び体外診断には使用できません。

Download SDS (MSDS)

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RIPA Lysis Buffer System (w/out PMSF)  参考文献

See how others have used RIPA Lysis Buffer System (w/out PMSF). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 22 total

PMID: # 31068616  Myszor, IT.|Parveen, Z.|Ottosson, H.|Bergman, P.|Agerberth, B.|Strömberg, R.|Gudmundsson, GH.| et al. 2019. Sci Rep. 9: 7114.

PMID: # 29868502  Cruz, R. et al. 2018. Front Cell Infect Microbiol. 8: 146.

PMID: # 26810190  Tong, WW. et al. 2016. Tumour Biol.

PMID: # 26342052  Shao, Y. et al. 2015. J Toxicol Sci. 40: 817-28.

PMID: # 25646597  Zhao, C. et al. 2015. International journal of oncology. 46: 1643-50.

PMID: # 25041469  Shi, SH. et al. 2015. Am. J. Reprod. Immunol. 73: 301-12.

PMID: # 25821818  Pająk, B. et al. 2015. BioMed research international. 2015: 746092.

PMID: # 25669938  Zhang, L. et al. 2014. Nature genetics. 46: 726-30.

PMID: # 24199849  Duan, X. et al. 2014. Clin Radiol. 69: 186-93.

PMID: # 23867821  Dufour, M. et al. 2013. Biochem. Biophys. Res. Commun. 438: 32-7.

Citations 1 to 10 of 22 total
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