p-p53 Antibody (3H2806): sc-71786

p-p53 Antibody (3H2806) is a mouse monoclonal IgG1 antibody that detects p53 in mouse, rat, human, porcine, and canine samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). p-p53 monoclonal antibody (3H2806) specifically recognizes the phosphorylated form of p53, a crucial tumor suppressor protein that plays a vital role in cellular stress responses by regulating the expression of genes involved in growth arrest and apoptosis. The phosphorylation of p53 enhances stability and transcriptional activity, allowing p53 to respond to DNA damage and other stress signals. This post-translational modification is essential for activating tumor-suppressive functions, as phosphorylation facilitates the transition from a latent to an active conformation, enabling DNA binding and target gene transcription initiation. Target genes include critical regulators such as p21, which enforces cell cycle arrest by inhibiting cyclin-dependent kinases, and pro-apoptotic factors like BAX and PUMA, which mediate programmed cell death. Through controlling the expression of these downstream effectors, p53 orchestrates a precise cellular response to prevent damaged DNA propagation, highlighting phosphorylation′s importance in maintaining genomic integrity. Since mutations in p53 are frequently associated with various human cancers, detecting and quantifying phosphorylated p53 using anti-p53 antibody (3H2806) is invaluable for research into cancer biology and therapeutic interventions.

The preferred p53 antibody is p53 Antibody (DO-1): sc-126.