OX2R Lentiviral Activation Particles (m): sc-425439-LAC

Cd200r1 encodes OX2R (CD200R1), an inhibitory immunoreceptor predominantly expressed on myeloid-lineage cells, including macrophages, dendritic cells, and microglia. Engagement by its ligand CD200 dampens inflammatory signaling and helps maintain tissue immune homeostasis by limiting activation programs downstream of innate immune receptors and cytokine pathways. OX2R-mediated regulation influences antigen-presenting cell function, phagocytic responses, and the balance of pro- versus anti-inflammatory gene expression in peripheral tissues and the central nervous system. Dysregulated CD200–CD200R1 signaling has been linked to altered neuroinflammatory states and immune-mediated pathology, making Cd200r1 a useful target for mechanistic studies of inflammation control.

OX2R Lentiviral Activation Particles (m) address this need by packaging the complete synergistic activation mediator (SAM) transcriptional activation system into transduction-ready, high-titer lentiviral particles, enabling efficient Cd200r1 upregulation across a broader range of human cell types.

OX2R Lentiviral Activation Particles (m) deliver all functional components of the synergistic activation mediator (SAM) system via lentiviral transduction. The system comprises three particle preparations co-transduced into target cells: one encoding catalytically inactive dCas9 (D10A and N863A mutations) fused to the VP64 transactivation domain with a blasticidin resistance gene; one encoding the MS2-p65-HSF1 fusion protein with a hygromycin resistance gene; and one encoding a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers with a puromycin resistance gene. Following lentiviral transduction and genomic integration of the expression cassettes, the SAM components are stably expressed and assemble at the target locus within the proximal promoter region upstream of the Cd200r1 transcriptional start site, where VP64, p65, and HSF1 act cooperatively to recruit endogenous transcriptional machinery and drive sustained upregulation of endogenous OX2R expression. The use of nuclease-inactive dCas9 avoids the introduction of double-strand DNA breaks and preserves the native Cd200r1 genomic locus and regulatory architecture.

The lentiviral format offers several practical advantages: stable genomic integration supports heritable activation across cell divisions; high-titer particle preparations eliminate the need for in-house viral production; and compatibility with primary, non-dividing, and transfection-resistant cell types expands experimental accessibility. Successful transduction can be confirmed and enriched through triple antibiotic selection using puromycin, hygromycin, and blasticidin.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.