OSR1 CRISPR/Cas9 KO Plasmid (h): sc-406034
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- OSR1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the OSR1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: OSR1 Antibody (C-8): sc-376545
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OSR1 CRISPR/Cas9 KO Plasmid (h) | sc-406034 | 20 µg | $397.00 |
Overview
OSR1 (odd-skipped related transcription factor 1) encodes a zinc finger transcription factor that regulates cell fate decisions and tissue patterning during embryonic development. In human cells, OSR1 influences transcriptional programs linked to mesenchymal differentiation, organogenesis, and extracellular matrix organization, with downstream effects on processes such as epithelial–mesenchymal interactions and morphogenesis. Dysregulated OSR1 expression has been associated with developmental abnormalities and has been reported in studies of tumor biology where altered differentiation and stromal programs contribute to disease phenotypes. As a nuclear DNA-binding protein, OSR1 is commonly investigated for its role in transcriptional regulation and lineage specification pathways.
OSR1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the OSR1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the OSR1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the OSR1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish OSR1 protein expression.
This CRISPR knockout system enables efficient generation of OSR1-deficient cell models for investigation of OSR1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting OSR1 exon(s) critical for OSR1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple OSR1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by OSR1 CRISPR/Cas9 KO Plasmid (h) and OSR1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the OSR1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by OSR1 HDR Plasmid (h) and OSR1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by OSR1 homology arms to support homology-directed repair at defined OSR1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.