Omni-probe Antibody (D-8): sc-7270

Omni-probe Antibody (D-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects the Omni-probe by western blotting (WB) and immunoprecipitation (IP). Omni-probe (D-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Omni-probe plays a crucial role in identifying and purifying hybrid fusion proteins, which are often generated using plasmid vectors that express coding regions of eukaryotic genes in various host systems such as E. coli, insect, and mammalian cells. These vectors typically encode hybrid proteins that combine prokaryotic and eukaryotic elements, allowing for efficient production and purification of proteins of interest. The presence of polyhistidine sequences in these fusion proteins enables rapid purification using nickel-charged agarose resin, making Omni-probe (D-8) monoclonal antibody an essential tool for researchers working with expressed fusion proteins. By targeting specific vector sequences located between the polyhistidine tag and the cDNA insert, Omni-probe (D-8) antibody facilitates convenient identification of expressed proteins across different host systems, thereby streamlining the research process and enhancing experimental outcomes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Omni-probe Antibody (D-8) References:

1. Versatile macrolide-responsive mammalian expression vectors for multiregulated multigene metabolic engineering.  |  Weber, W., et al. 2002. Biotechnol Bioeng. 80: 691-705. PMID: 12378611
2. Baculovirus expression vectors that incorporate the foreign protein into viral occlusion bodies.  |  Je, YH., et al. 2003. Biotechniques. 34: 81-7. PMID: 12545544
3. Assembly of functional GABAA receptors in insect cells using baculovirus expression vectors.  |  Atkinson, AE., et al. 1992. Neuroreport. 3: 597-600. PMID: 1330062
4. Improved bicistronic mammalian expression vectors using expression augmenting sequence element (EASE).  |  Aldrich, TL., et al. 1998. Cytotechnology. 28: 9-17. PMID: 19003402
5. Preparation of mammalian expression vectors incorporating site-specifically platinated-DNA lesions.  |  Ang, WH., et al. 2009. Bioconjug Chem. 20: 1058-63. PMID: 19351185
6. Co-expression vs. co-infection using baculovirus expression vectors in insect cell culture: Benefits and drawbacks.  |  Sokolenko, S., et al. 2012. Biotechnol Adv. 30: 766-81. PMID: 22297133
7. Mammalian expression vectors for metabolic biotinylation tandem affinity tagging by co-expression in cis of a mammalian codon-optimized BirA biotin ligase.  |  Ioannou, M., et al. 2018. BMC Res Notes. 11: 390. PMID: 29898783
8. Recent Developments in the Use of Baculovirus Expression Vectors.  |  Possee, RD., et al. 2020. Curr Issues Mol Biol. 34: 215-230. PMID: 31167962
9. Construction of gateway-compatible baculovirus expression vectors for high-throughput protein expression and in vivo microcrystal screening.  |  Tang, Y., et al. 2020. Sci Rep. 10: 13323. PMID: 32770037
10. ATP-dependent nucleosome disruption at a heat-shock promoter mediated by binding of GAGA transcription factor.  |  Tsukiyama, T., et al. 1994. Nature. 367: 525-32. PMID: 8107823
11. Phosphorylation and activation of a high molecular weight form of phospholipase A2 by p42 microtubule-associated protein 2 kinase and protein kinase C.  |  Nemenoff, RA., et al. 1993. J Biol Chem. 268: 1960-4. PMID: 8380583
12. Neutrophil phospholipase D is activated by a membrane-associated Rho family small molecular weight GTP-binding protein.  |  Bowman, EP., et al. 1993. J Biol Chem. 268: 21509-12. PMID: 8408000

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Omni-probe Antibody (D-8) has 59 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Omni-probe Antibody (D-8) Specifications

Alternate Product Name: Omni-probe

Product Clone Name: D-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 8-30

Epitope Target: Xpress series of prokaryotic and eukaryotic exspression vectors

Epitope Species:

Product Reactivity/Detection: fusion proteins encoded by the prokaryotic pRSET A, B, C and pTrc His A, B, C; baculovirus pBlue Bac His A, B, C and mammalian pEBV His A, B, C expression vectors

Species Reactivity/Detection: n/a

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 59 citations in various scientific publications

Available Conjugates/Forms for Omni-probe Antibody (D-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin