Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NIPBL Double Nickase Plasmid (m): sc-427895-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NIPBL Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NIPBL Double Nickase Plasmid (m) and NIPBL Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Nipbl. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NIPBL Antibody (C-9): sc-374625
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NIPBL Double Nickase Plasmid (m) | sc-427895-NIC | 20 µg | $410.00 | |||
NIPBL Double Nickase Plasmid (m2) | sc-427895-NIC-2 | 20 µg | $410.00 |
Nipbl encodes NIPBL, a cohesin-loading factor that coordinates sister chromatid cohesion and higher-order chromatin organization during the cell cycle. In mouse cells, NIPBL supports long-range enhancer–promoter communication and transcriptional control programs that govern embryonic development, lineage specification, and DNA damage responses. Disruption of Nipbl perturbs cohesin-dependent genome architecture and can influence replication stress signaling, chromosome segregation, and gene expression networks. Because altered NIPBL function is linked to developmental gene dysregulation and genome stability defects, it is a useful target for studying cohesinopathies and chromatin-associated disease mechanisms in vivo and in vitro models.
NIPBL Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Nipbl locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Nipbl. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Nipbl function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Nipbl-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.