Nek9 Antibody (39-7): sc-100401

Nek9 Antibody (39-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects Nek9 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Nek9 antibody (39-7) is available as non-conjugated format. Nek9, also known as NIMA (never in mitosis gene a)-related kinase 9, plays a crucial role in regulating cell cycle progression, particularly during G1/S transition and S phase, by influencing spindle dynamics and chromosome separation. This regulation is vital for maintaining genomic stability and ensuring proper cell division, which is essential for normal development and tissue homeostasis. With a length of 979 amino acids, Nek9 is predominantly localized in cytoplasm and nucleus, and shows high expression in liver, heart, kidney, and testis, while maintaining lower levels in fibroblasts and smooth muscle cells. Nek9 undergoes phosphorylation on serine and threonine residues, which is a key mechanism for modulating activity and interactions with other proteins. Notably, Nek9 interacts with several important proteins, including Ran GTPase, Nek6, Nek7, BICD2, SSRP1, and SUPT16H/FACT complex, highlighting an integral role in cellular processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Nek9 Antibody (39-7) References:

1. Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression.  |  Roig, J., et al. 2002. Genes Dev. 16: 1640-58. PMID: 12101123
2. Never say never. The NIMA-related protein kinases in mitotic control.  |  O'Connell, MJ., et al. 2003. Trends Cell Biol. 13: 221-8. PMID: 12742165
3. A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases.  |  Belham, C., et al. 2003. J Biol Chem. 278: 34897-909. PMID: 12840024
4. Nek9, a novel FACT-associated protein, modulates interphase progression.  |  Tan, BC. and Lee, SC. 2004. J Biol Chem. 279: 9321-30. PMID: 14660563
5. Active Nercc1 protein kinase concentrates at centrosomes early in mitosis and is necessary for proper spindle assembly.  |  Roig, J., et al. 2005. Mol Biol Cell. 16: 4827-40. PMID: 16079175
6. Caught Nek-ing: cilia and centrioles.  |  Quarmby, LM. and Mahjoub, MR. 2005. J Cell Sci. 118: 5161-9. PMID: 16280549

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Nek9 Antibody (39-7) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-Nek9 Antibody (39-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Nek9 Antibody (39-7) Specifications

Alternate Product Name: Nek9

Product Clone Name: 39-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 226-326

Epitope Target: Nek9

Epitope Species: human

Product Reactivity/Detection: Nek9

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for Nek9 Antibody (39-7): N/A