NCOAT Antibody (G-12): sc-376429

NCOAT Antibody (G-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects NCOAT protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). NCOAT (G-12) antibody is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NCOAT protein, also known as nuclear cytoplasmic O-GlcNAcase and acetyltransferase, or MGEA5 (meningioma-expressed antigen 5), is a bifunctional enzyme that plays a crucial role in cellular regulation by acting as both a β-hexosaminidase and a histone acetyltransferase. NCOAT is predominantly expressed in the placenta, brain, and pancreas, where NCOAT catalyzes the removal of O-GlcNAc residues from glycoproteins, thereby influencing various signaling pathways and cellular processes. NCOAT′s ability to acetylate specific lysine residues on histones H3 and H4 highlights a significant role in gene expression regulation through modifications of the histone code. NCOAT enzymatic activity is optimal at a slightly acidic pH of 5.7-7, and NCOAT function can be inhibited by free N-acetylglucosamine, which underscores the importance of substrate availability in modulating activity. NCOAT is expressed as three isoforms due to alternative splicing, with isoform 1 primarily localized in the cytoplasm and isoform 3 found in the nucleus, indicating a complex regulatory mechanism that may be critical for diverse biological functions.

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NCOAT Antibody (G-12) References:

1. Identification of a nuclear variant of MGEA5, a cytoplasmic hyaluronidase and a beta-N-acetylglucosaminidase.  |  Comtesse, N., et al. 2001. Biochem Biophys Res Commun. 283: 634-40. PMID: 11341771
2. Dynamic O-glycosylation of nuclear and cytosolic proteins: further characterization of the nucleocytoplasmic beta-N-acetylglucosaminidase, O-GlcNAcase.  |  Wells, L., et al. 2002. J Biol Chem. 277: 1755-61. PMID: 11788610
3. Analysis of MGEA5 on 10q24.1-q24.3 encoding the beta-O-linked N-acetylglucosaminidase as a candidate gene for type 2 diabetes mellitus in Pima Indians.  |  Farook, VS., et al. 2002. Mol Genet Metab. 77: 189-93. PMID: 12359146
4. Assignment of N-acetyl-D-glucosaminidase (Mgea5) to rat chromosome 1q5 by tyramide fluorescence in situ hybridization (T-FISH): synteny between rat, mouse and human with Insulin Degradation Enzyme (IDE).  |  Van Tine, BA., et al. 2003. Cytogenet Genome Res. 103: 202B. PMID: 15008141
5. Characterization of the histone acetyltransferase (HAT) domain of a bifunctional protein with activable O-GlcNAcase and HAT activities.  |  Toleman, C., et al. 2004. J Biol Chem. 279: 53665-73. PMID: 15485860
6. A single nucleotide polymorphism in MGEA5 encoding O-GlcNAc-selective N-acetyl-beta-D glucosaminidase is associated with type 2 diabetes in Mexican Americans.  |  Lehman, DM., et al. 2005. Diabetes. 54: 1214-21. PMID: 15793264
7. The histone acetyltransferase NCOAT contains a zinc finger-like motif involved in substrate recognition.  |  Toleman, CA., et al. 2006. J Biol Chem. 281: 3918-25. PMID: 16356930
8. Disrupting the enzyme complex regulating O-GlcNAcylation blocks signaling and development.  |  Whisenhunt, TR., et al. 2006. Glycobiology. 16: 551-63. PMID: 16505006
9. Location and characterization of the O-GlcNAcase active site.  |  Toleman, C., et al. 2006. Biochim Biophys Acta. 1760: 829-39. PMID: 16517082
10. Hypoxia-induced alterations in hyaluronan and hyaluronidase.  |  Gao, F., et al. 2005. Adv Exp Med Biol. 566: 249-56. PMID: 16594159
11. TGFBR3 and MGEA5 rearrangements in pleomorphic hyalinizing angiectatic tumors and the spectrum of related neoplasms.  |  Carter, JM., et al. 2014. Am J Surg Pathol. 38: 1182-992. PMID: 24705316
12. TGFBR3 and MGEA5 rearrangements are much more common in 'hybrid' hemosiderotic fibrolipomatous tumor-myxoinflammatory fibroblastic sarcomas than in classical myxoinflammatory fibroblastic sarcomas: a morphological and fluorescence in situ hybridization study.  |  Zreik, RT., et al. 2016. Hum Pathol. 53: 14-24. PMID: 26980036

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NCOAT Antibody (G-12) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-NCOAT Antibody (G-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NCOAT Antibody (G-12) Specifications

Alternate Product Name: NCOAT

Product Clone Name: G-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-300

Epitope Target: NCOAT

Epitope Species: human

Product Reactivity/Detection: NCOAT

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for NCOAT Antibody (G-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin