MYH1/2/3 Antibody (N3.36): sc-53092

MYH1/2/3 Antibody (N3.36) is a mouse monoclonal IgM kappa light chain antibody engineered to detect myosin heavy chain proteins encoded by the MYH1, MYH2, and MYH3 genes, corresponding to MyHC-IIx, MyHC-IIa, and MyHC-embryonic isoforms, respectively. These myosin heavy chains are crucial components of the sarcomeric thick filaments within skeletal muscle cells, where their hexameric structure, comprising two heavy chains and four light chains, enables interaction with actin to generate the force necessary for muscle contraction. The intricate structure of MYH1, MYH2, and MYH3 is vital for determining the contraction speed and functional properties of different muscle fiber types, making anti-MYH1/2/3 antibody (N3.36) an essential tool for investigating muscle physiology and development. MYH1/2/3 (N3.36) antibody is reactive across multiple species, including mouse, rat, human, rabbit, and fish, and is recommended for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). By facilitating the precise detection and analysis of various myosin heavy chain isoforms, MYH1/2/3 monoclonal antibody (N3.36) supports advanced research into muscle development, muscle disease mechanisms, and the molecular dynamics that drive muscle function.

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MYH1/2/3 Antibody (N3.36) References:

1. Structural implications of β-cardiac myosin heavy chain mutations in human disease.  |  Colegrave, M. and Peckham, M. 2014. Anat Rec (Hoboken). 297: 1670-80. PMID: 25125180
2. A missense mutation in MYH1 is associated with susceptibility to immune-mediated myositis in Quarter Horses.  |  Finno, CJ., et al. 2018. Skelet Muscle. 8: 7. PMID: 29510741
3. Generation of Functional Myocytes from Equine Induced Pluripotent Stem Cells.  |  Amilon, KR., et al. 2018. Cell Reprogram. 20: 275-281. PMID: 30207795
4. Myosin heavy chain-embryonic regulates skeletal muscle differentiation during mammalian development.  |  Agarwal, M., et al. 2020. Development. 147: PMID: 32094117
5. Myosin heavy chain expression relationships to power-load and velocity-load curves.  |  Lane, MT. and Fry, AC. 2021. J Sports Med Phys Fitness. 61: 2-9. PMID: 33092319
6. Nonmuscle myosin heavy chain IIA facilitates SARS-CoV-2 infection in human pulmonary cells.  |  Chen, J., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 34873039
7. α-myosin heavy chain lactylation maintains sarcomeric structure and function and alleviates the development of heart failure.  |  Zhang, N., et al. 2023. Cell Res. 33: 679-698. PMID: 37443257
8. Musculoskeletal defects associated with myosin heavy chain-embryonic loss of function are mediated by the YAP signaling pathway.  |  Bharadwaj, A., et al. 2023. EMBO Mol Med. 15: e17187. PMID: 37492882
9. The mammalian myosin heavy chain gene family.  |  Weiss, A. and Leinwand, LA. 1996. Annu Rev Cell Dev Biol. 12: 417-39. PMID: 8970733
10. C-terminal isoforms of the myosin heavy chain and smooth muscle function.  |  Martin, AF., et al. 1997. Comp Biochem Physiol B Biochem Mol Biol. 117: 3-11. PMID: 9180009

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MYH1/2/3 Antibody (N3.36) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-MYH1/2/3 Antibody (N3.36) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MYH1/2/3 Antibody (N3.36) Specifications

Alternate Product Name: MYH1/2/3

Product Clone Name: N3.36

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids:

Epitope Target: neonatal skeletal muscle Myosin

Epitope Species: human

Product Reactivity/Detection: myosin heavy chains encoded by MYH1, 2 and 3

Species Reactivity/Detection: mouse, rat, human, rabbit, fish

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for MYH1/2/3 Antibody (N3.36): N/A

The preferred MYH3 antibody is MYH3 Antibody (F1.652): sc-53091.