Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MDMX Double Nickase Plasmid (h): sc-417855-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MDMX Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MDMX Double Nickase Plasmid (h) and MDMX Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MDM4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MDMX Antibody (G-10): sc-74467
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MDMX Double Nickase Plasmid (h) | sc-417855-NIC | 20 µg | $410.00 | |||
MDMX Double Nickase Plasmid (h2) | sc-417855-NIC-2 | 20 µg | $410.00 |
MDM4 encodes MDMX, a key negative regulator of the TP53 tumor suppressor pathway that modulates p53-dependent transcriptional programs controlling cell-cycle arrest, apoptosis, and DNA damage responses. MDMX binds p53 and cooperates with MDM2 to restrain p53 activity, thereby influencing checkpoint signaling and cellular stress tolerance. Altered MDM4 expression or MDMX function has been associated with dysregulated p53 signaling in multiple cancer-relevant contexts and with impaired genome surveillance mechanisms. As a result, MDM4 is widely studied for its role in oncogenic pathway rewiring, replication stress responses, and p53 network robustness.
MDMX Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MDM4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MDM4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MDM4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MDM4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.