MCM8 CRISPR/Cas9 KO Plasmid (h): sc-413655
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MCM8 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MCM8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MCM8 CRISPR/Cas9 KO Plasmid (h) | sc-413655 | 20 µg | $397.00 |
Overview
MCM8 encodes a conserved minichromosome maintenance protein that forms a functional complex with MCM9 to promote DNA replication completion and homologous recombination–mediated repair, particularly during recovery from replication stress. MCM8 contributes to DNA end resection and the processing of stalled or collapsed replication forks, linking it to genome stability maintenance across S phase and meiotic recombination contexts. Disruption of MCM8 function has been associated with impaired double-strand break repair, increased chromosomal instability, and susceptibility to replication-associated DNA damage. Genetic alterations in MCM8 have been reported in infertility and premature ovarian insufficiency and are also relevant to mechanistic studies of tumorigenesis driven by defective DNA repair pathways.
MCM8 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MCM8 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MCM8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MCM8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MCM8 protein expression.
This CRISPR knockout system enables efficient generation of MCM8-deficient cell models for investigation of MCM8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MCM8 exon(s) critical for MCM8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MCM8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MCM8 CRISPR/Cas9 KO Plasmid (h) and MCM8 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MCM8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MCM8 HDR Plasmid (h) and MCM8 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MCM8 homology arms to support homology-directed repair at defined MCM8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.